LncRNA MEG3在病毒性毛细支气管炎患儿外周血单个核细胞和痰液中表达及意义

Expression of LncRNA MEG3 in Peripheral Blood Mononuclear Cells and Sputum of Children with Viral Bronchiolitis and Its Significance

目的:通过检测病毒性毛细支气管炎患儿外周血单个核细胞和痰液中LncRNA MEG3表达,分析其对疾病临床意义。方法:选取2017年2月至2019年1月上海市松江区九亭医院和上海市松江区中心医院收治的病毒性毛细支气管炎患儿103例,另选取同期体检显示健康婴幼儿103例为对照组。采集或收集患儿外周血单个核细胞和痰液后用qRT-PCR法检测LncRNA MEG3表达,分析其与患儿临床病理特征及预后关系。结果:对照、轻度、中度和重度组患儿外周血单个核细胞和痰液中LncRNA MEG3表达水平依次显著降低,两两比较差异有统计学意义(P均<0.05);对照、轻度、中度和重度组患儿外周血单个核细胞和痰液中VEGF mRNA表达水平依次显著升高,两两比较差异有统计学意义(P均<0.05)。患儿外周血单个核细胞中及痰液中LncRNA MEG3表达水平与VEGF mRNA表达水平均呈负相关(P均<0.05)。有过敏史及治疗出院后并发哮喘患儿外周血单个核细胞及痰液中LncRNA MEG3表达水平均显著低于无过敏史及未并发哮喘患儿(P<0.05)。有过敏史、VEGF高表达、LncRNA MEG3低表达是影响病毒性毛细支气管炎患儿发生不良预后危险因素(P均<0.05)。结论:对照、轻度、中度和重度组患儿外周血单个核细胞和痰液中LncRNA MEG3表达水平依次显著降低,可能与病毒性毛细支气管炎发生发展及患儿预后有关。

Objective:To detect the expression of LncRNA MEG3 in peripheral blood mononuclear cells and sputum of children with viral bronchiolitis and analyze its clinical significance.Methods:103 children with viral bronchiolitis admitted to Jiuting hospital and Songjiang Central Hospital from February 2017 to January 2019 were selected,and 103 healthy infants and children in the same period of physical examination were selected as the control group.After collecting peripheral blood mononuclear cells and sputum,the expression of LncRNA MEG3 was detected by qRT-PCR,and its relationship with clinicopathological characteristics and prognosis was analyzed.Results:The expression of LncRNA MEG3 in peripheral blood mononuclear cells and sputum of children in control,mild,moderate and severe groups decreased significantly in turn,and the differences between any two groups were statistically significant(P<0.05).The expression level of vascular endothelial growth factor(VEGF)mRNA in peripheral blood mononuclear cells and sputum of children in control,mild,moderate and severe groups increased significantly in turn,and the differences between any two groups were statistically significant(all P<0.05).The expression level of LncRNA MEG3 in peripheral blood mononuclear cells and sputum was negatively correlated with the expression level of VEGF mRNA(all P<0.05).The expression of LncRNA MEG3 in peripheral blood mononuclear cells and sputum of children with allergic history and post-discharge asthma was significantly lower than that of children without allergic history and concurrent asthma(P<0.05).Allergic history,high expression of VEGF and low expression of LncRNA MEG3 were risk factors for adverse prognosis in children with viral bronchiolitis(all P<0.05).Conclusions:The expression of LncRNA MEG3 in peripheral blood mononuclear cells and sputum decreased significantly in control,mild,moderate and severe groups,which may be related to the occurrence and development of viral bronchiolitis and the prognosis of children.