Ki-67、CK5/6、P53、P63的表达与食管鳞癌发生发展及临床病理特征的相关性

Expressions of Ki-67,CK5/6,P53 and P63 and Their Correlations with the Occurrence,Development and Clinicopathological Characteristics of Esophageal Squamous Cell Carcinoma

目的探讨Ki-67、细胞角蛋白5/6(CK5/6)、P53、P63的表达与食管鳞癌发生发展及临床病理特征的相关性。方法回顾性分析2017年1月至2019年4月安阳市肿瘤医院及郑州大学附属肿瘤医院病理中心确诊的3200例食管癌患者的病历资料,最终539例纳入研究。采用免疫组化法检测患者肿瘤组织中Ki-67、CK5/6、P53、P63的表达,并分析其与患者临床病理特征的相关性。结果食管鳞癌和食管非鳞癌患者神经侵犯、脉管癌栓、淋巴结转移、TNM分期、组织学分级、CK5/6、Ki-67、P63比较,差异有统计学意义(均P<0.05)。在食管鳞癌中,P53与TNM分期有关(P<0.05),与年龄、性别、脉管癌栓、神经侵犯、淋巴结转移、组织学分级均无关(均P>0.05);Ki-67、CK5/6、P63与年龄、性别、脉管癌栓、神经侵犯、淋巴结转移、TNM分期、组织学分级均无关(均P>0.05)。Spearman相关分析显示,CK5/6与Ki-67、P53与P63、CK5/6与P53、CK5/6与P63、Ki-67与P53、Ki-67与P63在食管鳞癌中的表达均呈正相关(均P<0.05)。结论联合检测Ki-67、CK5/6、P63可指导临床鉴别食管鳞癌与食管非鳞癌,检测P53可用于食管鳞癌预后评估及危险分级。

Objective To explore the expressions of Ki-67,cytokeratin5/6(CK5/6),P53 and P63 and their correlations with the occurrence,development and clinicopathological characteristics of esophageal squamous cell carcinoma.Methods The medical records of 3200 patients with esophageal cancer diagnosed by Pathology Center of Anyang Cancer Hospital and Zhengzhou University Cancer Hospital from January 2017 to April 2019 were retrospectively analyzed.A total of 539 patients with esophageal cancer were finally included in the study.The expression levels of Ki-67,CK5/6,P53 and P63 in the tumor tissues were detected by immunohistochemistry.The correlations between the expressions of Ki-67,CK5/6,P53,P63 and the clinicopathological characteristics of the patients were analyzed.Results There were statistical differences on nerve invasion,vascular tumor thrombus,lymph node metastasis,TNM stage,histological grade,CK5/6,Ki-67,and P63 between patients with esophageal squamous cell carcinoma and non-esophageal squamous cell carcinoma(all P<0.05).In esophageal squamous cell carcinoma,P53 was related to TNM stage(P<0.05),and it was not related to age,gender,vascular tumor thrombus,nerve invasion,lymph node metastasis and histological grade(all P>0.05).Ki-67,CK5/6,P63 had no correlations with age,gender,vascular tumor thrombus,nerve invasion,lymph node metastasis,TNM stage and histological grade(all P>0.05).Spearman correlation analysis showed CK5/6 and Ki-67,P53 and P63,CK5/6 and P53,CK5/6 and P63,Ki-67 and P53,Ki-67 and P63 were all positively correlated in esophageal squsquous cell carcinoma(all P<0.05).Conclusion Combined detection of Ki-67,CK5/6 and P63 can guide clinical differentiation of esophageal squamous cell carcinoma and non-squamous cell carcinoma,and detection of P53 can be used for prognosis evaluation and risk grading of esophageal squamous cell carcinoma.