牛大力内生细菌的多样性及其促生特性研究

Study on the Diversity and Growth Promoting Characteristics of the Endophytic Bacteria Isolated from Callerya speciose

为研究牛大力Callerya speciose内生细菌多样性组成及其促生特性,从经表面消毒处理的牛大力根茎组织中分离内生细菌,采用16SrDNA序列分析法对内生细菌的多样性进行系统发育分析,并以固氮、溶磷性、产铁载体、产IAA能力等为指标,对内生细菌进行促生特性检测。从牛大力根和茎中共分离到59株内生细菌,包括16属20种,芽孢杆菌属是牛大力的优势菌群;在20种分离菌株中,分别有11,17,7株具有潜在的固氮、溶磷性、产铁载体能力,有13株菌可以合成IAA。其中RH17-2、RH17-5、GR2-1 3株菌同时具备固氮、溶磷性、产铁载体、产IAA 4种促生特性。从牛大力中分离得到的内生细菌具有丰富的物种多样性和促生特性,在未来的农业生产上具有潜在的应用价值。

To explore the diversity composition of endophytic bacteria isolated from Callerya speciose,endophytes were isolated from the rhizome tissue of Cs.peciose that was sterilized on the surface.The diversity of endophytic bacteria was chosen to make phylogenetic analysis for the genetic diversity by 16 SrDNA sequence analysis,and the ability of nitrogen-fixation,phosphorus dissolution,siderophore production and IAA production were used as indicators to detect the growth promoting properties of endogenous bacteria.The results showed that a total of 59 strains of endophytic bacteria,including 20 species of 16 genera,were isolated from the roots and stems of Callerya speciose,and Bacillus were dominant bacteria.Among the 20 strains,11,17 and 7 strains had the potential of nitrogen-fixation,phosphorus solubilization and siderophore production,and13 strains could synthesize IAA.Among them,RH17-2,RH17-5 and GR2-1 strains had four growth-promoting characteristics of nitrogen-fixation,phosphorus solubilization,siderophore production and IAA production.In summary,the endophytic bacteria isolated fromCs.peciose have rich species diversity and growth promoting characteristics,and have potential application value in future agricultural production.