蛋白激酶A活化对血小板聚集功能的影响

Effect of Protein Kinase A Activation on Aggregation Function of Platelets

目的:研究蛋白激酶A(Protein kinase A,PKA)对体外血小板聚集功能的影响。方法:收集健康成年人外周血并获取洗涤血小板;采用PKA活化剂Forskolin体外处理洗涤血小板;利用瑞斯托霉素(Ristocetin),凝血酶(Thrombin),胶原(Collagen),二磷酸腺苷(ADP)诱导血小板聚集,利用血小板聚集仪检测血小板聚集情况。结果:与对照组相比较,5μmol/L的Forskolin可显著抑制ADP和Collagen诱导的血小板聚集(P<0.001);对Thrombin诱导的血小板聚集也具有一定抑制作用(P<0.05)。而2.5-10μmol/L的Forskolin对Ristocetin诱导的血小板聚集无明显抑制作用(P>0.05);对ADP和Collagen诱导的血小板聚集也具有显著抑制作用(P<0.001)。结论:蛋白激酶A活化能抑制血小板的聚集功能。

Objective:To investigate the effect of protein kinase A(PKA)activation on aggregation funetion of platelets in vitro.Methods:The peripheral blood of healthy adults were collected,and the washed platelets were gained from collected peripheral blood.The washed platelets were treated with PKA activator Forskolin,then the platelet aggregation was induced by using Ristocetin,Thrombin,Collagen and ADP respectively,the platelet aggregation level was detected by the platelet aggregator.Results:Compared with the controls,5μmol/L forskolin significantly inhibited ADP and collagen-induced platelet aggregation(P<0.001),and showed mild inhibiting effect on Thrombin-induced platelet aggregation(P<0.05).2.5-10μmol/L forskolin significantly inhibited ADP and Collagen-induced platelet aggregation(P<0.001);but not showed significantly inhibitory effects on Ristocetin-induced platelet aggregation(P>0.05).Conclusion:PKA activation inhibits agonists-induced platelet aggregation.