四极杆碰撞反应池ICP-MS同时测定贻贝中的Mo等12种重金属

Simultaneous determination of molybdenum and other heavy metals in Mytilus edulis by inductively coupled plasma mass spectrometry with quadrupole collision cell technology

运用微波密闭消解预处理样品,采用四极杆碰撞反应池技术(CCT)-电感耦合等离子体质谱法(ICP-MS)同时测定贻贝中Mo、V、Cr、Mn、Fe、Co、Ni、Cu、Zn、As、Cd和Pb等12种重金属,应用碰撞反应池碰撞模式(KED模式)引起的动能歧视效应,有效地消除了多原子离子对待测元素的质谱干扰,选用低中高质量数的45Sc、89Y、115In、209Bi混合内标更有效地校正基体效应和信号漂移,构建确定了测定的优化条件和技术流程。结果表明,KED-ICP-MS用于测定贻贝中重金属元素的含量结果令人满意,对12种重金属元素的相对标准偏差(RSD)为0.66%~3.91%,回收率在86.2%~102.0%之间,检出限在0.001×10^-9~0.118×10^-9之间,检测结果具有较高的准确度和精密度。该方法简便、快速、准确,可作为贻贝及其他海洋生物样品中重金属含量测定的理想方法,并可为海洋生物食品质量控制和安全评价提供科学依据。

The method for simultaneous determination of 12 heavy metals including Mo,V,Cr,Mn,Fe,Co,Ni,Cu,Zn,As,Cd and Pb in Mytilus edulis was established by ICP-MS with quadrupole collision cell technology,and using microwave digestion technique.The application of kinetic energy discrimination(KED) could eliminate the interference of polyatomic ions effectively.45Sc,89Y,115In and 209Bi as internal standard elements were also used to compensate matrix effect and signal drift,and the optimized conditions and technical procedures of the determination were established.The method of KED-ICP-MS for determination of heavy metals in Mytilus edulis was satisfactory,the relative standard deviations were between 0.66% and 3.91%,the recovery rate of the method was in the range of 86.2%~102.0%,and the detection limit ranged from 0.001×10^-9 to 0.118×10^-9.The results indicated that the method was convent,sensitivity,accurate and precision.It was suitable for determination of these elements in marine organisms,and also provided scientific foundation for safety assessment and quality control.