益气养阴方对宫颈癌大鼠免疫功能及癌组织的影响

Effects of Yiqi Yangyin Formula on Immune Function and Cancer Tissues of Cervical Cancer Rats

目的:探讨益气养阴方对宫颈癌大鼠免疫功能及癌组织P16、Ki67、P53、Survivin表达的影响。方法:采用右前腋下接种宫颈癌细胞U14的方法构建宫颈癌大鼠模型,将宫颈癌大鼠随机分为模型组、低剂量实验组和高剂量实验组,每组12只。低剂量实验组和高剂量实验组大鼠分别灌胃益气养阴方0.435、1.36 mL/kg/d,模型组灌胃等量生理盐水,连续干预30 d。采用流式细胞仪检测大鼠外周血免疫性T细胞亚群;采用HE染色与Wb法分别检测癌组织病理学变化与P16、Ki67、P53、Survivin蛋白表达。结果:与模型组比较,低剂量实验组和高剂量实验组大鼠外周血CD3+、CD4+、NKT细胞比例及癌组织P16、P53蛋白相对表达量均升高,高剂量实验组高于低剂量实验组;CD8+细胞比例、肿瘤重量与体积及癌组织Ki67、Survivin蛋白相对表达量均降低或缩小,高剂量实验组低于或小于低剂量实验组(P<0.05);且高剂量实验组的抑瘤率高于低剂量实验组(P<0.05)。结论:益气养阴方可有效上调宫颈癌大鼠癌组织P16、P53蛋白表达,下调Ki67、Survivin蛋白表达,从而抑制肿瘤生长,同时改善免疫功能,发挥治疗宫颈癌的作用。

Objective:To investigate the effects of Yiqi Yangyin Formula on the immune function and the expression of P16,Ki67,and P53,Survivin in cancer tissues of cervical cancer rats.Methods:The cervical cancer rat model was constructed by inoculating cervical cancer cells U14 in the right anterior armpit,and the cervical cancer rats were randomly divided into a model group and a low/high dose experimental group,each with 12 rats.Rats in the low/high-dose experimental group were given 0.435 and 1.36 mL/(kg·d)of Yiqi Yangyin Formula,respectively by gavage,and the model group was given the same amount of normal saline for 30 days.Flow cytometry was used to detect the peripheral blood immune T cell subsets in rats;HE staining and Wb method were used to detect the pathological changes of cancer tissues and the expression of P16,Ki67,P53,and Survivin protein,respectively.Results:Compared with the model group,the ratio of CD3+,CD4+,NKT cells and the relative expression of P16 and P53 proteins in the peripheral blood of rats in the low/high-dose experimental group were increased.The high-dose experimental group was higher than the low-dose experimental group;CD8+cell ratio,tumor weight and volume,and the relative expression of Ki67 and Survivin protein in cancer tissues were reduced or shrunk.The high-dose experimental group was lower or lower than the low-dose experimental group(P<0.05);and the tumor suppression rate of the high-dose experimental group was higher than that of the low-dose experimental group(P<0.05).Conclusion:Yiqi Yangyin Formula can effectively up-regulate the expression of P16 and P53 protein in cervical cancer tissues,and down-regulate Ki67,Survivin protein expression,inhibit tumor growth,improve immune function,and play a role in treating cervical cancer.