儿童血管瘤中miR-203与FGF-2的表达及意义

Relationship between expression of miR-203 and FGF-2 in children with hemangiomas and its significance

目的分析微小RNA-203(microRNA-203,miR-203)和成纤维细胞生长因子-2(fibroblast growth factors-2,FGF-2)在儿童血管瘤(hemangioma of infancy,HOI)中的表达水平并探讨其临床意义。方法选取2016年3月至2017年8月收治的55例HOI患者为HOI组,并将其分为增殖期(31例)与消退期(24例),另以术后正常组织标本为对照组(34例)。采用实时荧光定量PCR(qRT-PCR)检测各组miR-203与FGF-2 mRNA表达水平,免疫组织化学法检测FGF-2蛋白表达情况。观察的临床指标包括血管生长素(angiogenin,ANG)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、糖皮质激素受体α(glucocorticoid receptorα,GRα)、糖皮质激素受体β(glucocorticoid receptorβ,GRβ)。Pearson法比较分析HOI组患儿各指标间的相关性;Logistic多因素回归法分析HOI发生的相关影响因素。结果与对照组(1.01±0.15)相比,HOI组miR-203(0.73±0.24)表达水平显著降低(P<0.05),且增殖期(0.72±0.21)显著高于消退期(0.59±0.19)(P<0.05);HOI组FGF-2 mRNA(2.38±0.74)表达水平显著高于对照组(1.02±0.14)(P<0.05),且消退期(2.37±0.79)显著高于增殖期(2.03±0.68)(P<0.05);Pearson分析显示miR-203与FGF-2、ANG、VEGF、bFGF呈显著负相关(P<0.05),而FGF-2与之呈显著正相关(P<0.05);Logistic分析显示miR-203与FGF-2表达水平是HOI发生的影响因素。结论miR-203在HOI中低表达,而FGF-2呈高表达,两者在HOI分期中表达变化比较差异显著,对临床诊断HOI及治疗具有重要意义。

Objective To analyze the expression levels of microRNA-203(miR-203)and fibroblast growth factor-2(FGF-2)in hemangiomas of infancy(HOI)and to explore their clinical significance.Methods A total of 55 patients with HOI admitted to our hospital from March 2016 to August 2017 were selected as HOI group and divided into proliferative phase(31 cases)and regressive phase(24 cases),normal tissue specimens were taken as control group(34 cases).Real time fluorescence quantitative PCR(qRT-PCR)was used to detect the expression levels of miR-203 and FGF-2 mRNA in HOI tissues.Immunohistochemistry was used to detect the expression of FGF-2 protein in HOI tissues.Clinical indicators of HOI patients included angiogenin(ANG),vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),glucocorticoid receptor alpha(GRα),glucocorticoid receptor beta(GRβ).Pearson method was used to compare and analyze the correlation between various indicators in children with HOI group.Logistic multivariate regression analysis was used to analyze the related influencing factors of HOI.Results Compared with the control group(1.01±0.15),the expression level of miR-203 in HOI group(0.73±0.24)decreased significantly(P<0.05),and the proliferative phase(0.72±0.21)was significantly higher than the regression phase(0.59±0.19)(P<0.05);the expression levels of FGF-2 mRNA and protein in HOI group(2.38±0.74)were significantly higher than those in control group(1.02±0.14)(P<0.05),and the regression phase(2.37±0.79)was significantly higher than the proliferative stage(2.03±0.68)(P<0.05);Pearson analysis showed that miR-203 was negatively correlated with FGF-2,ANG,VEGF and bFGF(P<0.05),while FGF-2 was positively correlated with them(P<0.05);Logistic analysis showed that the expression levels of miR-203 and FGF-2 were both influencing factors of HOI.Conclusion MiR-203 is low expressed in HOI,while FGF-2 is highly expressed.There is a significant difference in the expression changes of the two in HOI stages and it is important for clinical diagnosis of HOI and treatment.