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反相高效液相色谱法测定神经酸片剂中神经酸含量 被引量:3

Determination of Nervonic Acid in Nervonic Acid Tablets by Reversed Phase High Performance Liquid Chromatography
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摘要 目的建立神经酸片剂中神经酸的反相高效液相色谱含量测定方法。方法色谱柱为UltimateR XB-C8 (4. 6 mm×250 mm,5μm),流动相为0.1%(V/V)磷酸水溶液-乙腈(93:7),检测波长为203 nm,流速1.0 m L/min,柱温25℃。结果神经酸与其他组分分离良好,神经酸浓度在0.01~0.2 mg/m L范围内线性关系良好(Y=3 000 000 X (mg/m L)+4 795.9,P=0.000,R2=0.999 9,n=5),该方法精密度实验RSD为1.80%(n=6)。样品溶液在24 h稳定,峰面积RSD为1.52%(n=6)。回收率为96.94%~104.46%(RSD在0.81%~3.80%之间)。结论首次采用C8柱,建立了一种反相高效液相色谱方法,实现了对片剂中神经酸含量的快速准确测定。 Objective To establish a RP-HPLC method for the determination of nervonic acid in nervonic acid tablets.Methods Ultimate XB-C8(4.6 mm×250 mm,5μm)column was used.The mobile phase consisted of 0.1%(V/V)phosphoric acid aqueous solution-acetonitrile(93:7).The detection wavelength was 203 nm.The flow rate was 1.0 mL/min.And the column temperature was 25℃.Results Nervonic acid peak was well separated from other components.Nervonic acid showed a good linear relationship in the concentration range of 0.01-0.2 mg/mL with the peak area(Y=3000000 X(mg/mL)+4795.9,R2=0.9999,n=5).The precision RSD%of this method was 1.80%,which met the requirements.The sample had good stability within 24 hours with a RSD%of 1.52%bystability test.The recovery rate was between 96.94%and 104.46%(RSD was between 0.81%and 3.80%).Conslusion For the first time,the C8 column was used in reversed phase high performance liquid chromatography to directly and accurately determine the content of nervonic acid in tablets.
作者 吴乐艳 侯雯清 孙孔春 杨璨瑜 舒纹 沈报春 WU Le-yan;HOU Wen-qing;SUN Kong-chun;YANG Can-yu;SHU Wen;SHEN Bao-chun(School of Pharmaceutical Science&Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming Yunnan 650500,China)
出处 《昆明医科大学学报》 CAS 2020年第6期11-14,共4页 Journal of Kunming Medical University
基金 国家自然科学基金资助项目(81860632)。
关键词 反相高效液相色谱法 神经酸 含量测定 High performance liquid chromatogrophy Nervonic acid Determination
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  • 1李全,刘复初,朱洪友,林军,王惠昌.Z-15-二十四碳烯酸的制备[J].云南化工,2001,28(6):34-36. 被引量:4
  • 2许松林,徐世民,干爱华.天然产物分离的新技术—分子蒸馏[J].中草药,2001,32(6):562-563. 被引量:32
  • 3王性炎,王姝清.神经酸新资源——元宝枫油[J].中国油脂,2005,30(9):62-64. 被引量:61
  • 4王性炎,樊金栓,王姝清.中国含神经酸植物开发利用研究[J].中国油脂,2006,31(3):69-71. 被引量:73
  • 5贺浪中 高雯.元宝枫油乳液对艾氏腹水癌小鼠的抗肿瘤作用[A]..元宝枫开发利用研究[C].西安:陕西科学技术出版社,1996..
  • 6BIRON C A, NGUYEN KB, PIEN G C, et al. Natural killer cells in antiviral defense:function and regulation by innate cytokines [J]. Annu Rev Immunol, 1999,17: 189 - 220
  • 7BREHM M A, SELIN L K, WELSH R M. CD8 T cell responses to viral infections in sequence [J]. Cell Microbiol, 2004,6(5 ) :411 - 421
  • 8RIDDEL S R,GRENBERG P D. Principles for adoptive T cell therapy of human viral diseases[J]. Annu Rev Immunol, 1995,13 : 545 - 586
  • 9DE LIBERO G, MORI L. Recognition of lipid antigens by T cells [ J ]. Nat Rev Immunol, 2005,5 (6) : 485 - 496
  • 10BOISSONNAS A, FETLER L, ZEELENBERG I S, et al. In vivo imaging of cytotoxic T cell infiltration and elimination of a solid tumor [ J ]. Exp Med,2007,204 (2) :345 - 356

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