基于皮克林乳液聚合四环素磁性分子印迹—生物炭微球的研制

Study on the preparation of tetracyline magnetic molecularly imprinted biochar microspheres based on Pickering emulsion polymerization

以四环素(TC)为模板分子,生物炭和四氧化三铁为皮克林乳液共稳定剂,通过皮克林乳液聚合法制备了对四环素类抗生素(TCs)具有特异性吸附的磁性分子印迹生物炭微球。通过吸附动力学试验、等温吸附试验及选择性吸附试验对微球的吸附性能进行了详细评价,结果表明印迹微球对TC具有良好的特异性吸附,印迹因子为3.79。将所得材料作为吸附剂用于实际样品中TCs的提取、净化,结合高效液相色谱对样品中TCs含量进行测定。在最优条件下,方法在5~160μg/L范围内线性关系良好,精密度为1.23%~9.99%,最低检出限(LOD)为1.42~1.58μg/L,实际样品的回收率为90.6%~103.4%,表明所建立的方法可以用于食品中TCs痕量残留的准确分析。

In this study,magnetic molecularly imprinted biochar microspheres for specific adsorption of tetracylines(TCs)were prepared by Pickering emulsion polymerization using tetracyline(TC)as template and Fe 3 O 4 and biochar as co-stabilizers.The adsorption properties of the imprinted microspheres for TC were studied by adsorption kinetics test,adsorption isothermal test,and selectivity adsorption test,exhibiting the excellent specific adsorption for TC with imprinting factor of 3.97.Further more,the imprinted materials were used as adsorbents for extraction and purification of TCs in real samples,and the content of TCs were detected through high performance liquid chromatography(HPLC).Under the control of the optimum conditions,the established method showed a good linearity in the range from 5μg/L to 160μg/L with the precision of 1.23%~9.99%,limits of detection(LOD)of 1.42~1.58μg/L,and high recoveries of 90.6%~103.4%.This indicated that the proposed method could be applied for the accurate analysis of TCs at trace level in food.