野地瓜不同部位活性成分、抗氧化能力及其相关性分析

Analysis of active compounds,antioxidant activity and correlation in different parts from Ficus tikoua Bur.

以贵州野地瓜为试验材料,分析其不同部位的酚类物质、VC、总酚、总黄酮含量,采用总还原力(TRPA)、ABTS+自由基清除能力(ABTS)和Fe^2+的还原能力(FRAP)法评价其体外抗氧化能力,并通过相关性分析来确定主要抗氧化成分。结果显示:藤、叶和果实中抗氧化活性物质含量及抗氧化能力存在显著差异(P<0.05)。藤的总酚和总黄酮含量显著高于叶和果实(P<0.05),而叶中V C含量显著高于藤和果实(P<0.05);各部位主要酚类成分为没食子酸、鞣花酸、绿原酸和芦丁,叶中酚类物质显著高于果实和藤(P<0.05);果实的FRAP显著高于ABTS和TRPA(P<0.05),藤和叶的ABTS和FRAP均显著高于TRPA(P<0.05),果实的ABTS和FRAP均显著高于藤和叶(P<0.05),且随着样品质量浓度的增加其抗氧化活性呈正相关;相关性分析发现,总酚对TRPA贡献最大,鞣花酸对FRAP贡献显著,绿原酸对ABTS有显著贡献。

To investigate the bioactive compounds and antioxidant activity of vine,leaves and fruits of the Ficus tikoua Bur.in Guizhou Province,the phenolic compounds,vitamin C,total phenols,total flavonoids were analyzed.Antioxidant activity was evaluated by total reducing power assay(TRPA),free radical scavenging capacity of ABTS^+·(ABTS)and ferric ion reducing antioxidant power(FRAP)method in vitro,and Pearson correlation analysis was conducted.The results indicated that the contents of bioactive compounds and antioxidant activity were significantly different between the vine,leaves and fruits(P<0.05).The content of total phenols and flavonoids in vine was significantly higher than that in leaves and fruits(P<0.05),while the content of V C in leaves was significantly higher than that in fruits and leaves(P<0.05).Gallic acid,ellagic acid,chlorogenic acid and rutin were the major phenolic compounds in the Ficus tikoua Bur.Phenolic compounds in leaves was significantly higher than that in fruits and vine(P<0.05).The FRAP of fruit was significantly higher than that of ABTS and TRPA(P<0.05).The ABTS and FRAP of vine and leaf were significantly higher than that of TRPA(P<0.05),ABTS and FRAP of fruit were significantly higher than those of vine and leaf(P<0.05).The antioxidant activity of the samples increased positively with the concentration.Relevance analysis found that total phenols has a strongest contribution to the TRPA value,and the ellagic acid and chlorogenic acid contribute significantly to the FRAP and ABTS,respectively.The Ficus tikoua Bur.can be fully exploited and utilized according to the content of bioactive compounds and antioxidant activity.