湖南常德地区草地贪夜蛾肠道细菌的鉴定及其寄主分析

Identification of the intestinal bacteria in Spodoptera frugiperda collected from Changde area and analysis of potential host plants

对湖南常德地区草地贪夜蛾(Spodoptera frugiperda)幼虫的肠道细菌进行分离培养,结合16Sr DNA测序,共分离得到11个细菌分离株,分别归为肠杆菌属(Enterobacter)、泛菌属(Pantoea)、莱略特氏菌属(Lelliottia)、芽孢杆菌属(Bacillus)、克雷伯氏菌属(Klebsiella)5个属,与分离自玉米粘虫(Mythimna separata)幼虫肠道细菌归属的肠杆菌属、芽孢杆菌属及克雷伯氏菌属在种类上相似;发现草地贪夜蛾幼虫在玉米、豇豆、辣椒和甘蓝等植物上均可存活,存活率分别为70%、50%、17%和10%,这些植物可能是湖南地区的潜在寄主。

The intestinal bacteria of larvae in Spodoptera frugiperda(S.frugiperda),that migrated in Changde area Hunan province,were isolated by traditional culture method and identified by 16S rDNA.In total,11 strains of bacteria were obtained,belonging to genus of Enterobacter,Pantoea,Lelliottia,Bacillus and Klebsiella.Enterobacter,Bacillus and Klebsiella identified from S.frugiperda are similar to the same genus isolated from the gut of larvae in Mythimna separata.S.frugiperda could survive in Zea mays,Vigna unguiculate,Capsicum annuum and Brassica oleracea,and the larval survival rates of S.frugiperda are 70%,50%,17%,10%,respectively.These plants could be the potential hosts of S.frugiperda in Hunan province.