多烯紫杉醇对前列腺癌细胞基因SPC24表达的影响

Effect of Docetaxel on Expression of Prostate Cancer Cell Gene SPC24

目的前列腺癌在中国的发病率逐年上升,其致病诱因包括种族、年龄、饮食、遗传等。SPC24是核分裂周期80(NDC80)复合物的亚基之一,参与细胞有丝分裂中的着丝点-纺锤体微管精准耦合、染色体精确均分等过程。目前前列腺癌的发病机制是否与基因SPC24表达的相关研究鲜有报道。本研究探究多烯紫杉醇(docetaxel,DT)对前列腺癌细胞基因SPC24表达的影响,探讨其成为潜在前列腺癌潜在治疗的生物标志物可能性。方法体外培养前列腺癌细胞DU145,随机分成实验组和阴性对照组。其中实验组加入含不同浓度DT(10^-8 mol/L、10^-7 mol/L、10^-6 mol/L、10^-5 mol/L)的培养基,阴性对照组加入等体积培养液。经干预24、48、72、96 h后,采用Cell Counting Kit-8(CCK8)法检测DT对前列腺癌细胞DU145增殖的影响,利用RT-qPCR和Western blotting检测技术分别检测SPC24 mRNA和SPC24蛋白的表达水平。结果DT可显著抑制DU145细胞增殖,且随DT浓度的增加抑制率逐渐升高,于72 h达到最高。选取抑制效果最佳的10^-5 mol/L浓度的DT作用72 h后,SPC24 mRNA表达水平显著降低(P<0.05),SPC24蛋白表达显著降低(P<0.05)。结论DT能抑制前列腺癌细胞DU145增殖,其作用机制可能与SPC24表达下调有关,SPC24可能是前列腺癌治疗的潜在靶点。

Objective The incidence of prostate cancer in China is increasing year by year,and its causative factors include race,age,diet,and genetics.SPC24 is one of the subunits of the Nuclear Division Cycle 80(NDC80)complex,and is involved in processes such as the precise coupling of centromere-spindle microtubules in cell mitosis and precise chromosome equipartition.At present,whether the pathogenesis of prostate cancer is related to the expression of gene SPC24 has not been reported.This study explored the effect of docetaxel(DT)on the expression of prostate cancer cell gene SPC24 and explored its potential to become a potential biomarker for potential prostate cancer treatment.Methods Prostate cancer cells DU145 were cultured in vitro and randomly divided into an experimental group and a negative control group.Among them,the experimental group added medium containing different concentrations of DT(10^-8 mol/L,10^-7 mol/L,10^-6 mol/L,10^-5 mol/L),and the negative control group added equal volume of culture medium.After 24,48,72,and 96 hours of intervention,Cell Counting Kit-8(CCK8)method was used to detect the effect of DT on the proliferation of prostate cancer cell DU145.RT-qPCR and Western blotting detection techniques were used to detect the expression levels of SPC24 mRNA and SPC24 protein,respectively.Results DT can significantly inhibit the proliferation of DU145 cells,and the inhibition rate gradually increases with the increase of DT concentration,reaching the highest at 72h.After 72 hours of DT with the best inhibitory effect of 10^-5 mol/L,the expression level of SPC24 mRNA was significantly reduced(P<0.05),and the expression of SPC24 protein was significantly reduced(P<0.05).Conclusion DT inhibits the proliferation of prostate cancer cell DU145,and its mechanism may be related to the down-regulation of SPC24 expression,which may be a potential target for prostate cancer treatment.