摘要
目的比较不同呼吸道试子保存液对核酸检测结果的影响,期望找到一种常温条件下实现拭子样本中的核酸稳定保存的保存液。方法采集427例呼吸道感染患儿咽拭子样本,同一患者采集两根,交替方式洗脱到两种不同保存液中。对两种样本保存液拭子进行QPCR检测,对比阳性检测率;结果康健拭子保存液保存样本,检出甲型流感病毒105例,阳性率24.59%,检测出呼吸道合胞病毒47例,阳性检出率11.01%,新型保存液保存样本,检测出甲型流感病毒104例,阳性率24.36%,检测出呼吸道合胞病毒46例,阳性检出率10.77%,两种保存液检出率无无显著性差异;结论新型保存液可常温保存运输拭子病毒样本,在分子诊断中有较高应用价值。
Objective To compare the effects of different respiratory tract preservation solutions on the results of nucleic acid detection,and to find a kind of preservation solution for stable nucleic acid preservation in swab samples at room temperature.Methods 427 samples of throat swabs from children with respiratory tract infection were collected.Two swabs were collected from the same patient and eluted alternately into two different preservation solutions.Results 105 cases of influenza A virus and 47 cases of respiratory syncytial virus were detected,and the positive rate was 11.01%.104 cases of influenza A virus were detected,the positive rate was 24.36%,and 46 cases of respiratory syncytial virus were detected.The positive detection rate was 10.77%.There was no significant difference between the two preservation solutions.Conclusion The new preservation solution can preserve the transport swab virus samples at room temperature and has high application value in molecular diagnosis.
作者
李晓燕
靳秀红
杨俊梅
LI Xiao-yan;JIN Xiu-hong;YANG Jun-mei(Children's hospital of zhengzhou university,zhengzhou 450000,Henan Province,China)
出处
《罕少疾病杂志》
2020年第4期20-21,47,共3页
Journal of Rare and Uncommon Diseases
基金
河南省医学科技攻关计划联合共建项目(2018020698)。
关键词
PCR
呼吸道
样品保存
PCR
Respiratory Tract
Sample Preservation