摘要
通过离子交换色谱法,建立不同厂家阿胶及动物皮胶中Cl-、SO42-、NO3-、PO43-4种阴离子的测定方法。结果表明,4种阴离子分离效果良好,Cl-、SO42-、NO3-、PO43-分别在0.000 922~0.023 1、0.001 09~0.027 3、0.000 167~0.004 18、0.000 314~0.007 86 mg/mL浓度范围内线性关系良好,平均加样回收率在98.7%~101.0%。该测定方法结果准确、灵敏、可靠,重现性较好,可用于阿胶及动物皮胶中4种阴离子含量的测定。
To establish a method to determine the contents of Cl-,SO42-,NO3-,PO43-in animal skin gelatin and E jiao from different manufacturers by IEC.Results showed that Cl-,SO42-,NO3-,PO43-were well separated and showed good linearity at 0.000922~0.0231,0.00109~0.0273,0.000167~0.00418,and 0.000314~0.00786 mg/mL.The average recoveries were between 98.7%~101.1%.Result of this method was accurate,sensitive,credible and repeatable.It could be applied to the content determination of 4 kinds of inorganic anions in animal skin gelatin and E jiao from different manufacturers.
作者
刘雯
李峰
覃洁萍
冯旭
LIU Wen;LI Feng;QIN Jie-ping;FENG Xu(College of Pharmacy,Guangxi University of Traditional Chinese Medicine,Nanning 530001,China;College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)
出处
《湖北农业科学》
2020年第8期134-137,共4页
Hubei Agricultural Sciences
关键词
阿胶
阴离子
离子交换色谱
含量
测定
E jiao
inorganic anionsion exchange chromatography
ion exchange chromatography(IEC)
content
determination
