固相微萃取/气相色谱-质谱法测定檀香香气成分

Determination of aroma components of Santalum album linn by solid phase microextraction-gas chromatography-mass spectrometry

采用固相微萃取法富集檀香(Santalum album linn)中挥发性成分,通过气相色谱-质谱联用技术对檀香香气成分进行分析,并通过面积归一法计算各成分的相对含量。结果表明,在常温干燥状态下,从檀香中共鉴定出39种化学成分,主要有烯烃类(91.15%)、烷烃类(3.00%)、醇类(2.56%)、酯类(2.19%)、酮类(0.55%)、醛类(0.41%)、杂环类(0.14%)。该方法具有样品用量少、易操作、快速鉴定香气成分且灵敏度高等优点,能有效分离测定出檀香中挥发性成分,实现对不同檀香品种的快速鉴别,为进一步研究中药材提供技术支持。

The volatile components of sandalwood were enriched by solid phase microextraction and analyzed by gas chromatography-mass spectrometry.The relative content of each component was calculated by area normalization method.The results showed that under normal temperature and drying condition,39 chemical constituents were identified,including olefins(91.15%),alkanes(3.00%),alcohols(2.56%),esters(2.19%),ketones(0.55%),aldehydes(0.41%)and heterocycles(0.14%).This method has the advantages of less sample consumption,easy operation,rapid identification of aroma components and high sensitivity.It could effectively separate and determine volatile substances in sandalwood,realize rapid identification of different sandalwood varieties,and provide effective technical support for further research of Chinese medicinal materials.