摘要
目的建立茵陈清筋颗粒(茵陈、垂盆草、蒲公英等)的质量标准。方法 TLC法定性鉴别茵陈、垂盆草、栀子、拳参、大黄、甘草、蒲公英,HPLC法测定没食子酸、绿原酸、咖啡酸、栀子苷含有量。结果 TLC斑点清晰,阴性无干扰。没食子酸、绿原酸、咖啡酸、栀子苷分别在10.16~101.6、24.94~249.4、1.38~13.84、39.69~396.9μg/mL范围内线性关系良好(r=0.999 9),平均加样回收率分别为100.30%、99.08%、98.63%、98.24%,RSD分别为1.3%、1.2%、1.9%、1.4%。结论该方法准确可靠,重复性好,专属性强,可用于茵陈清筋颗粒的质量控制。
AIM To establish the quality standard for Yinchen Qingjin Granules(Artemisiae scopariae Herba,Sedi Herba,Taraxaci Herba,etc.).METHODS TLC was adopted in the qualitative identification of Artemisiae scopariae Herba,Sedi Herba,Gardeniae Fructus,Bistortae Rhizoma,Rhei Radix et Rhizoma,Glycyrrhizae Radix et Rhizoma and Taraxaci Herba.HPLC was used for the content determination of gallic acid,chlorogenic acid,caffeic acid and geniposide.RESULTS The TLC spots were clear without negative interference.Gallic acid,chlorogenic acid,caffeic acid,geniposide showed good linear relationships within the ranges of 10.16-101.6,24.94-249.4,1.38-13.84,39.69-396.9μg/mL(r=0.9999),whose average recoveries were 100.30%,99.08%,98.63%,98.24%with the RSDs of 1.3%,1.2%,1.9%,1.4%,respectively.CONCLUSION This accurate,reliable,reproducible and specific method can be used for the quality control of Yinchen Qingjin Granules.
作者
陆杰
王婷婷
陈颖
王新宏
安叡
赵诚
LU Jie;WANG Ting-ting;CHEN Ying;WANG Xin-hong;AN Rui;ZHAO Cheng(College of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China)
出处
《中成药》
CAS
CSCD
北大核心
2020年第6期1437-1442,共6页
Chinese Traditional Patent Medicine
基金
上海市中医药三年行动计划(2018-2020)项目[ZY(2018-2020)-CCCX-4005]。