HPLC法同时测定茶芎苯酞类有效部位中5种成分

Simultaneous determination of five constituents in phthalide target areas of Lgus-ticum sinense by HPLC

目的建立HPLC法同时测定茶芎Lgusticum sinense Oliv.cv. Chaxiong.苯酞类有效部位中洋川芎内酯A、正丁基苯酞、新蛇床内酯、Z-藁本内酯、丁烯基苯酞的含有量。方法茶芎苯酞类有效部位样品甲醇提取物的分析采用AkzoNobel Kromasil?100-5-C18柱(4.6 mm×250 mm,5μm);流动相水-乙腈,梯度洗脱;体积流量1.0 mL/min;柱温30℃;洋川芎内酯A、Z-藁本内酯检测波长280 nm,正丁基苯酞、新蛇床内酯、丁烯基苯酞检测波长230 nm。结果洋川芎内酯A、正丁基苯酞、新蛇床内酯、Z-藁本内酯、丁烯基苯酞分别在18.08~289.34μg/mL(r=0.999 9)、1.02~16.40μg/mL(r=0.999 9)、8.32~133.11μg/mL(r=0.999 8)、21.71~347.36μg/mL(r=0.999 9)、1.14~18.22μg/mL(r=0.999 6)范围内线性关系良好,平均加样回收率分别为99.03%、98.25%、97.78%、99.35%、97.95%,RSD分别为0.33%、1.07%、0.33%、0.59%、1.01%。结论该方法稳定可靠,可用于茶芎苯酞类有效部位的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of senkyunolide A, N-butylphthalide, new osthol lactone, Z-ligustilide and butenyl phthalide in phthalide target areas of Lgusticum sinense Oliv.cv. Chaxiong.METHODS The analysis of methanol extract of pathalide target areas of Chaxiong was performed on a 30 ℃ thermostatic Kromasil 100-5-C18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of water-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength of senkyunolide A, Z-ligustilide was set at 280 nm,and it was 230 nm of N-butylphthalide, new osthol lactone and butenyl phthalide.RESULTS Senkyunolide A, N-butylphthalide, new osthol lactone, Z-ligustilide and butenyl phthalide showed good linear relationships within the ranges of 18.08-289.34 μg/mL(r=0.999 9),1.02-16.40 μg/mL(r=0.999 9),8.32-133.11 μg/mL(r=0.999 8), 21.71-347.36 μg/mL(r=0.999 9),1.14-18.22 μg/mL(r=0.999 6),whose average recoveries were 99.03%, 98.25%, 97.78%, 99.35% and 97.95% with the RSDs of 0.33%, 1.07%, 0.33%, 0.59% and 1.01%, respectively.CONCLUSION This stable and reliable method can be used for the quality control of phthalide target areas of Chaxiong.