HPLC法测定不同产地半夏及其伪品中4种核苷

Determination of four nucleosides in Pinellia ternata and its adulteration from different growing areas by HPLC

目的建立HPLC法测定不同产地半夏及其伪品中尿苷、肌苷、鸟苷和腺苷的含有量。方法半夏水溶液的分析采用Agilent ZORBAX SB-Aq C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱;体积流量1 mL/min;检测波长260 nm;柱温25℃。结果尿苷、肌苷、鸟苷、腺苷分别在0.002 0~0.040μg/mL(r=0.999 2)、0.002 4~0.048μg/mL(r=0.999 4)、0.032 3~0.323μg/mL(r=0.999 9)、0.002 9~0.058μg/mL(r=0.999 1)范围内线性关系良好;平均回收率(RSD)分别为100.49%(2.05%)、100.19%(1.16%)、98.99%(1.92%)、100.12%(1.65%)。结论该方法准确稳定,重复性好,可用于半夏的质量控制。

AIM To establish an HPLC method for content determination of uridine,inosine,guanosine and adenosine in Pinellia ternata(Thunb.)Breit.and its adulteration from different growing areas.METHODS The analysis of aqueous extract of P.ternata was performed on a 25℃thermostatic ZORBAX SB-AqC18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of andacetonitrile-water flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.RESULTS Uridine,inosine,guanosine and adenosine showed good linear relationships within the ranges of 0.0020-0.040μg/mL(r=0.9992),0.0024-0.048μg/mL(r=0.9994),0.0323-0.323μg/mL(r=0.9999),0.0029-0.058μg/mL(r=0.9991),whose average recoveries(RSD)were 100.49%(2.05%)、100.19%(1.16%)、98.99%(1.92%)、100.12%(1.65%),respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of P.ternata.