大气压冷等离子体对肿瘤细胞增殖和凋亡的影响及其机制

Effects of cold atmospheric plasma on the proliferation and apoptosis of tumor cells and its mechanisms

目的:探讨大气压冷等离子体(CAP)对人肿瘤细胞增殖和凋亡的影响。方法:将处于对数生长期的人神经胶质瘤细胞(U87)和宫颈癌细胞(Hela)接种于培养板或培养皿中,待细胞贴壁生长后,将其随机分为正常对照(control)组、氦气(He)组和CAP不同时间处理组,细胞在接受不同时间CAP处理后,采用CCK-8方法检测细胞活力,流式细胞仪检测细胞凋亡情况,蛋白印迹法检测凋亡相关蛋白caspase3和氧化应激相关蛋白Nrf2的表达水平。结果:He处理对U87和Hela细胞的增殖能力和自发凋亡无明显影响,CAP处理后两种肿瘤细胞的细胞活力均显著降低,凋亡水平明显上升,caspase3蛋白和Nrf2蛋白的表达显著增加,CAP处理时间越长,效应越明显。结论:本实验条件下的CAP处理可抑制人肿瘤细胞的增殖,并诱导其凋亡,其机制可能与CAP导致的氧化应激有关。

Objective To explore the effects of cold atmospheric plasma(CAP)on the proliferation and apoptosis of human tumor cells.Methods Human glioma cells(U87)and cervical cancer cells(Hela)which were in logarithmic growth phase were inoculated in culture plate or culture dish.When the cells grew adhering to the wall,they were randomly divided into control group,helium(He)group and CAP groups with different treatment durations.After different treatments,the cell viability was detected by CCK-8 method,and apoptosis was tested by flow cytometry,and the expression levels of caspase-3 protein related to apoptosis and Nrf2 protein related to oxidative stress were measured by Western blot.Results He treatment did not affect the proliferation and apoptosis of U87 cells and Hela cells.Compared with those in control group and He group,the viability of U87 cells and Hela cells in CAP group were significantly decreased,while the apoptosis rate was obviously increased.Moreover,the expression levels of caspase3 protein and Nrf2 protein in CAP group were remarkably increased,and the increase was more obvious with the longer duration of CAP treatment.Conclusion CAP can inhibit the proliferation of human tumor cells and induce apoptosis under this experimental condition,and the oxidative stress might be involved in this process.