分散固相萃取结合毛细管电泳-激光诱导荧光法测定血清中甲基化精氨酸

Determination of methylated arginines in serum by a dispersive solid-phase extraction coupled with capillary electrophoresis-laser induced fluorescence detection

介绍一种分散固相离子交换萃取方法,结合毛细管电泳-激光诱导荧光检测法,测定血清样品中不对称二甲基精氨酸、对称二甲基精氨酸和单甲基精氨酸。将提取甲基化精氨酸的树脂粒加入到4-氯-7-硝基苯并呋喃乙腈溶液和碱性硼酸钠溶液(pH=10.5)的混合溶液中,于60℃水浴中衍生化。在80 mmol/L磷酸盐溶液(pH=2.0)分离电解质和20 kV分离电压条件下,这3种衍生物在熔融石英毛细管中获得基线分离。根据FDA生物分析方法验证指南,进行方法验证,结果满意。利用该方法测定了当地医院血清样品中的3种甲基化精氨酸含量。

We describe a dispersive solid-phase ion exchange extraction method combined with capillary electrophoresis-laser induced fluorescence detection for the determination of asymmetric dimethylarginine,symmetric dimethylarginine,and monomethylarginine in serum samples.The methylated arginine-extracted resin beads were added into a mixed solution of 4-chloro-7-nitrobenzofuran acetonitrile solution and alkaline sodium borate solution(pH=10.5)for the derivatization in a 60℃water bath.The three derivatives were subjected to baseline separation in a fused silica capillary under the conditions of a separation electrolyte of 80 mmol/L phosphate(pH=2.0)and a separation voltage of 20 kV.On the basis of the FDA bioanalytical method validation guidance,the method validation was conducted and the results were satisfactory.This method was used to determine the three methylated arginines in the serum samples from local hospital.