促红素新型环性衍生肽对顺铂诱导肾小管上皮细胞损伤的保护作用

Protective effects of cyclic helix B peptide on cisplatin-induced renal tubular epithelial cell injury

目的 :探讨促红素新型环性衍生肽(cyclic helix B peptide,CHBP)对顺铂诱导肾小管上皮细胞(NRK-52E)损伤的保护作用及可能机制。方法:体外培养NRK-52E细胞,分为(1)对照组:NRK-52E细胞(4×105个)单独培养;(2)顺铂组:10μmol/L顺铂处理NRK-52E细胞;(3)CHBP干预组:顺铂处理NRK-52E细胞加入不同浓度CHBP干预,低浓度亚组为16 nmol/L CHBP,中浓度亚组为32 nmol/L CHBP,高浓度亚组为64 nmol/L CHBP。每个亚组分别干预12 h、24 h。采用流式细胞术及Annexin V-FITC-PI法检测NRK-52E细胞凋亡率。结果:顺铂组处理24 h凋亡率较12 h增高,差异具有统计学意义(P<0.05);在处理12 h和24 h两个时间点,顺铂组凋亡率高于对照组,差异均具有统计学意义(P<0.05);在处理12 h后,低浓度亚组凋亡率与顺铂组比较,差异无统计学意义(P>0.05),中、高浓度亚组的凋亡率低于顺铂组,差异均具有统计学意义(P<0.05);在处理24 h时间点,各干预组凋亡率低于顺铂组,差异均具有统计学意义(P<0.05)。结论:CHBP对顺铂诱导NRK-52E细胞损伤具有保护作用。

Objective:To investigate the effects of cyclic helix B peptide(CHBP)on cisplatin-induced renal tubular epithelial cells(NRK-52E)injury and possible regulatory mechanisms.Methods:NRK-52E cells culture in vitro was divided into three groups:(1)the control group:the NRK-52E cells(4×105)culture alone;(2)the cisplatin group:10μmol/L cisplatin treatment of NRK-52E cells;(3)the CHBP intervention group:1 low concentration subgroup:16 nmol/L CHBP+10μmol/L cisplatin treatment of NRK-52E cells;2 medium concentration subgroups:32nmol/L CHBP+10μmol/L cisplatin treatment NRK-52E cells;3 high concentration subgroups:64nmol/L CHBP+10μmol/L cisplatin Treatment of NRK-52E cells.Each subgroup was intervened for 12h and 24h respectively.Flow cytometry(FCM)was used to detect NRK-52E cells apoptosis:The apoptosis of NRK-52E cells was detected by Annexin V-FITC-PI method.Results:The degree of NRK-52E cells apoptosis in the CHBP intervention group was lighter than that in the cisplatin group,and it was positively correlated with concentration.P<0.05;Conclusion:CHBP has protective effects on cisplatin-induced NRK-52E cells injury.