利用体外发酵模型研究叶绿素对儿童肠道菌群的影响

Effects of Chlorophyll on Intestinal Flora from Children in Vitro Fermentation Model

通过不同年龄儿童粪便的体外发酵,研究叶绿素的生物代谢及对肠道菌群的影响,探明其生物活性,明确摄入绿叶蔬菜的健康意义。体外发酵试验菌群测序分析结果表明:叶绿素降低了儿童肠道菌群的多样性,添加叶绿素体外发酵24 h后,2岁儿童菌群多样性指数由2.6~3.2降至2.4~2.7,5岁儿童菌群多样性指数由2.5~2.8降至1.8~2.4。属水平上,柯林斯菌属、双岐菌属相对丰度降低,乳酸菌属的相对丰度升高。菌群代谢物检测结果表明,叶绿素促进同年龄儿童粪便代谢物乳酸、乙酸水平的增加,说明叶绿素具有一定的生物活性,影响肠道菌群组成及其代谢产物含量的变化。

This study investigated the biological metabolism of chlorophyll and its impact on the intestinal flora with the in vitro fermentation experiments to understand the health significance of intaking green-leafy vegetables.In the intestinal flora sequencing test of human fecal fermentation,the addition of chlorophyll reduced the diversity of flora.After 24 hours of fermentation experiments,in the chlorophyll group,the diversity index of two-years old child decreased from 2.6-3.2 to 2.4-2.7 and the diversity index of five-years old child decreased from 2.5-2.8 to 1.8-2.4.While in the field of genus,the relative abundance of Collinsella and Bifidobacterium were significantly reduced.In the change of the Bacterial metabolites,the increase of lactic acid,acetic acid was promoted by chlorophyll.Therefore,chlorophyll has a certain biological activity,which can affect the intestinal flora and its metabolites content changes.