采后硝普钠处理对生姜根茎能量代谢的影响

Effects of Sodium Nitroprusside Dipping Treatment on Energy Metabolism in Ginger Rhizomes

为探讨外源硝普钠(SNP)对生姜根茎能量代谢的影响,分别用0和0.25 mmol/L的SNP溶液浸泡生姜10 min,研究常温贮藏期间线粒体H+-ATPase、Ca^2+-ATPase、琥珀酸脱氢酶、细胞色素氧化酶活性、ATP、ADP和AMP含量及能荷的变化。结果表明,0.25 mmol/L的SNP处理提高了采后生姜根茎线粒体H^+-ATPase、Ca^2+-ATPase、琥珀酸脱氢酶、细胞色素氧化酶活性。SNP处理还延缓了生姜体内ATP和ADP含量及能荷的下降,使生姜根茎维持较高的能量水平,从而延缓生姜根茎采后衰老进程,更好地保持生姜根茎的品质。

To investigate the effects of sodium nitroprusside(SNP)treatment on energy metabolism in ginger rhizomes,ginger rhizomes was dipped in 0 and 0.25 mmol/L SNP for 10 min and then stored at room temperature.The activities of H^+-ATPase,Ca^2+-ATPase,succinate dehydrogenase(SDH)and cytochrome oxidase(CCO)in mitochondria,content of ATP,ADP,AMP and energy charge were determined during storage.The results showed that the activities of H+-ATPase,Ca2+-ATPase,SDH and CCO were enhanced by SNP treatment.SNP treatment also delayed the decrease of ATP and ADP contents and energy charge in mitochondria of ginger rhizomes.These results suggested that SNP treatment can maintain higher energy levels through regulation the activity of enzymes in energy metabolism to delay the senescence and keep the quality of ginger rhizomes.