人髓样分化因子88的生物信息学分析

Bioinformatics Analysis of MyD88 in Human

人髓样分化因子88(MyD88)属于Toll样受体和IL-1受体家族成员下游的炎症信号通路的中心因子。为了研究人MyD88的蛋白结构和功能,本文利用重要数据库进行了生物信息学分析。研究表明,人MyD88基因全长为5670 bp,位于染色体3p22.2,共编码296个氨基酸。人MyD88蛋白具有较高的保守性,其等电点为5.64,不存在信号肽,并且含有2个苏木化位点、15个丝氨酸磷酸化位点、6个苏氨酸磷酸化位点和4个酪氨酸磷酸化位点。该蛋白主要定位在细胞核和细胞质中,而且在血液、脾脏和肺较多,其二级结构含有145个α-螺旋和30个β-折叠,拉曼图表明三级结构模型A是可信的。与人MyD88相互作用的蛋白主要是Toll样受体和白介素相关蛋白,并且参与炎症反应、细胞凋亡等重要的免疫过程。本研究为阐明人MyD88基因结构特点和生物学功能奠定了理论基础,也为其相关疾病诊断和治疗提供了新思路。

Human myeloid differentiation factor 88(MyD88)is the central factor for inflammatory signaling pathways downstream of members of the Toll-like receptor(TLR)and interleukin-1(IL-1)receptor families.To explore the human MyD88 structures and functions by bioinformatics analysis,results showed human MyD88 gene of full length 5670 bp,located on chromosome 3p22.2,and encoded 296 amino acids,which was a higher conserved one.Meanwhile,human MyD88 was highly distributed in the blood,spleen and lung,with no signal peptides,whereas it included 2 sumolation sites and 15 serine phosphorylation sites,6 threonine phosphorylation sites and 4 tyrosine phosphorylation sites.The secondary structure was mainly consisted of 145α-helix and 30β-sheet regions,indicating that the tertiary structure was reliable by ramachandran plot.In addition,human MyD88 also has protein interaction with Toll-like receptors and interleukins,participating in inflammatory reaction,cell apoptosis and other immunologic processes.All together,our bioinformatics analysis would provide a basis for understanding structure features and biological functions of human MyD88,guiding a new schema for the diagnosis and treatment in the future.