脂肪干细胞对于同种异体脂肪移植免疫排斥反应的抑制作用

Inhibition of ADSC on the Immune Rejection of Adipose Allotransplantation

目的探究脂肪干细胞ADSC(adipose-derived stem cells)对于同种异体脂肪移植排斥反应的抑制作用。方法以BAL/BC小鼠作为供体,以C57BL/6J小鼠作为受体进行同种异体脂肪移植实验后,提取移植的脂肪组织,通过HE(苏木精-伊红)染色、BODIPY染色以及脂周蛋白(lipoprotein)免疫荧光染色等方式观察移植物中脂肪细胞的存活状况。然后在相同的实验中,在移植物当中混合含有成球BAL/BC小鼠(供体)脂肪干细胞的HA(透明质酸)凝胶作为变量,检测移植物中的脂肪细胞存活状况,与之前的结果进行对比。结果当在同种异体脂肪移植实验当中,在移植的脂肪组织中加入脂肪干细胞后,成活的脂肪细胞明显高于无脂肪干细胞参与的对照组。结论脂肪干细胞对于同种异体脂肪移植排斥反应有一定的抑制作用。

Objective To explore the inhibitory of the rejection of the ADSC(adipose-derived stem cells)in the adipose allotransplantation.Methods Through using the BAL/BC mouse as a donor and the C57 BL/6 J mouse as a receptor to conduct the experiment of the adipose allotransplantation,the transplanted adipose tissue was extracted.The survival of the transplanted adipose cells through the hematoxylineosin staining,BODIPY staining and the lipoprptein imminofluorescent staining,etc was observed.After that,the implant that mixed with the HA(Hyaluronic Acid)gel which have the balling ADSC of BAL/BC mouse(the donor)were regarded as the variable,to test the survival of the adipose cell in the implant,and to make contrast with the previous result.Resuls In the experiment of the adipose allotransplantation,the survival adipose cell evidently exceeds the contrast group without the ADSC after putting the ADSC in the transplanted adipose tissue.Conclusion There are some inhibitory actions of rejection that the ADSC takes to the adipose allotransplantation.