武夷岩茶对酒精性肝损伤大鼠的护肝作用

The Protective Effect of Wuyi Rock Tea on Rats with Alcoholic Liver Injury

为研究武夷岩茶对酒精性肝损伤大鼠的肝脏保护作用,将试验分成5组,正常对照组(Normal),酒精肝模型组(Model),武夷岩茶低、中、高剂量组(L、M、H),每组清洁级SD雄性大鼠12只;除Normal组外,其余各组大鼠以7 g/(kg·d)酒精灌胃,同时武夷岩茶L、M、H组分别以剂量为0.75、1.50、3.00 g/(kg·d)的武夷岩茶制成的茶汤灌胃。第8周末,检测肝脏组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛(MDA),检测肝功能ALT、AST、甘油三酯(TG),取肝组织HE染色行病理检查。与Model组相比,武夷岩茶各剂量组均可降低大鼠血清肝功能指标AST、ALT(P<0.05),武夷岩茶H组可降低大鼠血清TG(P<0.05),武夷岩茶H、M组可提高肝组织中GSH-Px、SOD且降低MDA(P<0.05),病理结果示武夷岩茶H、M剂量组肝组织损伤明显减轻。可见中、高剂量武夷岩茶对酒精性肝损伤大鼠具有护肝作用。

To investigate the protective effect of Wuyi rock tea on rats with alcoholic liver injury,sixty sprague-dawley rats were randomly divided into normal control,model and Wuyi rock tea treatment(0.75,1.5,3.0 g/(kg·d)groups,12 cases in each group.Except for the normal control group,rats in the other groups were by alcohol intragastric administration with 7 g/(kg·d)alcohol,while groups with the low,middle and high dose of Wuyi rock tea were by intragastric administration with Wuyi rock tea soup in doses of 0.75,1.5 and 3.0 g/(kg·d)respectively.For eight weeks,the ALT,AST and GST levels in serum and SOD,MDA and GSH-Px levels in liver tissue were detected.Liver injury was assessed by pathological examination.Results Compared with the model group,each dose group of Wuyi rock tea could reduce the serum AST and ALT(P<0.05),the high dose group could reduce the serum TG(P<0.05),and the middle and high dose group could reduce MDA and increase SOD and GSH-Px in liver tissue(P<0.05).Pathological examination showed that liver tissue injury was significantly reduced in Wuyi rock tea groups.Thus it can be seen that Wuyi rock tea with middle and high dose has a protective effect on the rat liver with alcoholic injury.