响应面法优化甘草酸超声提取工艺研究

Optimization of ultrasonic extraction of glycyrrhizic acid by response surface method

目的研究甘草酸的超声最佳提取工艺参数。方法以料液比、提取时间及提取温度为主要研究因素,以提取的甘草酸含量为响应值,先利用单因素确定3个水平的拐点,再用Box-Behnken实验设计不同水平实验,运用Design-Expert 10.0.3软件对测得的结果建立二次回归模型。结果通过对单因素实验的分析,确定料液比3个水平分别取1:50、1:65、1:80(g/mL)。提取温度分别取20、25、30℃3个水平,提取时间分别取2、5、8 min 3个水平,Box-Behnken试验结果得甘草酸超声提取工艺最佳参数应为料液比为1:59(g/mL),提取温度25.5℃,提取时间5.7 min时能取得最大值。结论经过试验反复验证,所得到的实测值与预测值吻合程度良好,即说明响应面模型具有较好的预测能力,能够有效用于甘草酸提取值的预测。

Objective To study the optimal ultrasonic extraction parameters of glycyrrhizic acid.Methods With the ratio of material to liquid,extraction time and extraction temperature as the main research factors,and the extracted glycyrrhizic acid content as the response value,three inflection points at three levels were determined by single factor,and then Box-Behnken was used to design experiments at different levels.A quadratic regression model was established for the measured results by using the design-expert 10.0.3 software.Results Through the analysis of single factor experiment,three levels of material-liquid ratio were determined:1:50,1:65 and 1:80(g/mL),respectively.The extraction temperature was set at 20,25 and 30℃,and the extraction time was set at 2,5 and 8 min,respectively.The box-behnken test results showed that the optimal ultrasonic extraction parameters of glycyrrhizic acid were the ratio of material to liquid 1:59(g/mL),the extraction temperature was 25.5℃,and the extraction time was 5.7 min.Conclusion The experimental results show that the measured value is in good agreement with the predicted value,which indicates that the response surface model has a good prediction ability and can be effectively used for the prediction of glycyrrhizic acid extraction value.