超高效液相色谱法检测方便面中叶黄素

Determination of the lutein in instant noodle by ultra performance liquid chromatography

目的建立超高效液相色谱检测方便面中叶黄素的方法。方法方便面样品中的叶黄素用石油醚提取,涡旋离心,过膜,供超高效液相色谱检测。紫外检测器检测波长为445 nm,采用外标法定量。结果叶黄素在0.5~10.0μg/mL浓度范围内线性关系良好,相关系数大于0.9945,方法检出限为0.1 mg/kg,平均回收率为82.1%~105.3%,相对标准偏差为6.5%~12.2%。结论本方法操作简便、经济实用、结果准确可靠,可用于方便面中叶黄素的批量检测。

Objective To establish a method for determination of lutein in instant noodle by ultra performance liquid chromatography(UPLC).Methods The lutein in the instant noodle was extracted with petroleum ether,vortexed for centrifugation,and then passed through a membrane for ultra performance liquid chromatography.The detection wavelength of ultraviolet detector was 445 nm,and the external standard method was used for quantitative analysis.Results The linear relationship of lutein was good in the range of 0.5-10.0 μg/mL with correlation coefficients above0.9945.The detection limit of the method was 0.1 mg/kg and the recovery rate was 82.1%-105.3%,with the relative standard deviation(RSD) 6.5%-12.2%.Conclusion The method is simple,economical and practical,and the result is accurate and reliable,which is suitable for batch determination of lutein in instant noodle.