红茂草异紫堇碱对小鼠实验性肝脏损伤的保护作用

Protective effects of isocorydine alkaloid in Dicranostigma Leptopodum(Maxim.)Fedde(DLF)on experimental liver injuries

研究红茂草异紫堇碱对小鼠实验性肝损伤的保护作用。给予小鼠不同剂量的红茂草异紫堇碱灌胃10 h,后腹腔注射四氯化碳(CCl4)诱导小鼠急性肝损伤病理模型,16 h后测定小鼠肝指数、小鼠血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、肝脏中丙二醛(MDA)的含量及超氧化物歧化酶(T-SOD)的变化。采用环磷酰胺诱导小鼠免疫性肝损伤病理模型,用不同浓度的异紫堇碱灌服10 h,测定小鼠肝指数、血清中IL-1β、IL-2、INF-γ和TNF-α含量变化,同时进行肝脏组织结构和病理变化观察。结果发现,与模型组相比,不同剂量的异紫堇碱给药组血清中AST、ALT活性显著降低(P<0.01),肝组织匀浆MDA含量显著减少(P<0.01);抗氧化酶T-SOD活性显著升高(P<0.01);血清中IL-2和INF-γ含量显著升高(P<0.01),病理切片显示红茂草异紫堇碱作用后肝组织损伤有不同程度地减轻,高剂量的异紫堇碱(330.96 mg/kg)对肝组织的保护程度最为明显。说明红茂草异紫堇碱对实验性肝损伤具有很好的保护作用,其机制与DLF调节细胞因子水平,抑制抗氧化酶活性,清除自由基,抑制脂质过氧化相关。

The research aimed to investigate the effects of isocorydine alkaloid in DLF on experimental liver injurie in mice.Different dosage of isocorydine alkaloid in DLF(82.74 mg·kg-1,165.48 mg·kg-1 and 330.96 mg·kg-1)was injected though stom-ach in mice and CCl 4 was given though intraperitoneal injection for 10 d continuously to cause the acute liver injury.After 16 h,the activities of aspartate aminotransferase(AST),and alanine am inotransferase(ALT)in serum,and malondialdehyde(MDA)concentration and the activities of superoxide dismutase(SOD)in liver plasma,and liver index were detected.In addition,to dis-cuss the effects of isocorydine alkaloid on immunological liver injury induced by cyclophosphamide in mice.Isocorydine alka-loid-treated group was intragastrically treated with different bodyweight of isocorydine alkaloid described above for 10 days.After 16 h,the content of IL-1β,IL-2,INF-γand TNF-αin serum were determined.At the same time,the pathological observa-tion of liver tissues was made.The pretreatment of isocorydine alkaloid in DLF markedly reduced the serum AST and ALT activ-itie(P<0.01).The increase of hepatic MDA in CCl 4-treated mice was partially prevented by DLE pretreatment(P<0.01).The ac-tivities of hepatic SOD in isocorydine alkaloid-treated mice were significantly increased(P<0.01).The serum IL-2 and INF-γwere significantly increased(P<0.01).Isocorydine alkaloid pretreatment of 330.96 mg·kg-1 bodyweight induced a marked bene-ficial effect on the prevention of liver injury as demonstrated morphologically that the liver damage was significantly decreased.The results showed isocorydine alkaloid in DLF had protective effects on the experimental liver injury induced by CCl 4 and cy-clophosphamide in mice.The mechanisms may contribute to isocorydine alkaloid in DLF antioxidant activities and its effects of removing free radicals,suppressing lipid peroxidation of membrane and decreasing the level of MDA.Furthermore,it may be related to isocorydine alkaloid in DLF regulation of cytokine levels.