库仑滴定法快速测定吡拉西坦的含量研究

Determination of the Content of Piracetam by Coulometric Titration

利用库仑滴定法快速测定吡拉西坦的含量。铂片电极分别作电解电极对和指示电极对,0.8 mol/L溴化钾(pH 9.0)为支持电解质溶液,经20.00 mA恒电流电解生成BrO-后,与预处理后的样品溶液中NH+4定量发生氧化还原反应,记录电解至滴定终点时间,根据法拉第电解定律,计算样品中吡拉西坦的含量。利用该方法分别测定不同厂家的样品,结果与HPLC法和凯氏定氮法相近,无显著性差异(P>0.05),且相对标准偏差均低于1.60%,同时该方法操作简便、快速,因而适用于吡拉西坦的质量检测。

A novel method that coulometric titration to rapidly determine content of piracetam was described.Two couple of platinum electrode were served as working electrode pairs and indicating electrode pairs respectively.0.8 mol/L potassium bromide solution(pH 9.0)acted as the support electrolyte solution,and intensity of constant current was 20.00 mA.The BrO-electrolytic generated by the potassium bromide solution electrolysis reacted with the NH+4 in the pretreatment sample solution,which was quantitative oxidation-reduction reaction.The result was obtained by Faraday′s law through recording time that titrated to titration end-point.The piracetam of three brands were seperately measured by coulometric titration,HPLC method,and kjeldahl method.The results showed that the three methods result had no significant difference(P>0.05),and the relative standard deviations of coulometric titration result were lower than 1.60%.Meanwhile,the proposed method is simple,convenience,rapid,and so on,then will be suit for the piracetam content determination.