慢性乙肝病毒感染引起的肝硬化患者血清microRNA表达变化研究

Study the SerummicroRNA Expressions Change in Chronic HBV-Induced Liver Cirrhosis

目的:探讨慢性乙肝病毒感染引起的肝硬化患者血清microRNA表达变化及临床意义。方法:按照一定标准对某院2017年1月~2018年10月间因慢性乙肝病毒感染引起的肝硬化患者进行筛选,共62例入选,并采集血液样品,离心收集血清,冻-80℃备用,同时选取30例正常人作为对照。通过分析代偿期及失代偿期的肝硬化病人microRNA表达变化,分析异常表达的microRNA对肝硬化病人的预后价值。结果:肝硬化代偿组与失代偿组患者microRNA低于对照组,其中肝硬化代偿组低于失代偿组,microRNA表达高于失代偿组,差异有统计学意义(P<0.05)。结论:microRNA作为一类新的标记物,在肝硬化过程中具有重要作用,血清microRNA的表达变化可以作为肝硬化检测的指标,为肝硬化的临床诊断、治疗和预后提供重要的参考依据。

Objective:To study the serum microRNA expressions change and clinical significance in chronic HBV(hepatitis b virus)-induced liver cirrhosis.Methods:According to screening standards,62 chronic HBV-induced liver cirrhosis patients treated from January 2017 to October 2018 in a hospital were selected.The blood samples were collected.After centrifugation,serum was obtained and stored at-80℃.30 healthy check-ups were selected as control group.Serum microRNA expressions at compensated stage and decompensated stage were detected.The clinical significance of abnormal microRNA expressions in patient's prognosis was analyzed.Results:Serum microRNA expressions at compensated stage and decompensated stage in liver cirrhosis group were lower than those in the control group;among liver cirrhosis group,related expressions at compensated stage were higher than those at decompensated stage(P<0.05).Conclusion:microRNA is a new marker that plays a vital role in liver cirrhosis.Serum microRNA expressions can be taken as a detection marker of liver cirrhosis and provide the basis on clinical diagnosis,treatment and prognosis assessment.