茵陈四苓颗粒对胆汁淤积性肝炎大鼠肝功能损害的改善作用及其机制研究

Improvement of liver injuries by herbal medicine in rats with ANTT-induced cholestatic hepatitis

目的探讨茵陈四苓颗粒对胆汁淤积性肝炎大鼠肝功能损害的改善作用及可能的机制。方法将30只SD大鼠随机分为对照组、模型组和茵陈四苓颗粒干预组,采用α-异硫氰酸萘酯(ANIT)灌胃法制备胆汁淤积性肝炎大鼠模型。采用RT-PCR法检测肝组织白介素-17(IL-17)、干扰素-γ(IFN-γ)、超氧化物歧化酶(SOD)和法尼酯X受体(FXR)mRNA水平。结果茵陈四苓颗粒干预组动物血清ALP和GGT水平分别为(254.3±16.4)U/L和(14.3±5.8)U/L,显著低于模型组【分别为(625.8±36.5)U/L和(26.7±7.9)U/L,P<0.05】;血清IL-17、IFN-γ和丙二醛水平分别为(162.2±16.4)pg/ml、(28.9±6.2)pg/ml和(19.2±3.8)U/ml,显著低于模型组【分别为(223.4±18.8)pg/ml、(39.3±9.8)pg/ml、(28.6±2.4)U/ml,P<0.05】,而血清SOD水平为(324.1±33.5)U/ml,显著高于模型组【(250.8±25.4)U/ml,P<0.05】;茵陈四苓颗粒干预组肝组织IL-17和IFN-γmRNA水平为(2.1±0.3)和(17.3±3.5),显著低于模型组【分别为(3.4±0.5)和(34.5±7.2),P<0.05】,而SOD和FXR mRNA水平为(0.8±0.4)和(2.2±0.5),显著高于模型组【分别为(0.3±0.2)和(1.5±1.1),P<0.05】。结论茵陈四苓颗粒能明显改善胆汁淤积性肝炎大鼠肝组织损伤,其机制可能与调控IL-17、IFN-γ和MDA等因子水平,降低炎症反应和提高抗氧化能力有关。

Objective The aim of this study was to investigate the effect of Yinchen Siling granule,a herbal medicine,on the improvement of liver injuries in rats with alpha-naphthylisothiocyanate(ANIT)-induced cholestatic hepatitis.Method Thirty SD rats were randomly divided into control,model and herbal medicine-intervened group.The interleukin(IL)-17,interferon-γ(IFN-γ),superoxide dismutase(SOD),and farneside x receptor(FXR)mRNA in liver tissues were assayed.Results Serum ALP and GGT levels in herb-intervened group were(254.3±16.4)U/L and(14.3±5.8)U/L,significantly lower than【(625.8±36.5)U/and(26.7±7.9)U/L,respectively,P<0.05】in the model;serum IL-17,IFN-γand malondialdehyde levels were(162.2±16.4)pg/ml,(28.9±6.2)pg/ml and(19.2±3.8)U/ml,significantly lower than[(223.4±18.8)pg/ml,(39.3±9.8)pg/ml and(28.6±2.4)U/ml,respectively,P<0.05],while serum SOD level was(324.1±33.5)U/ml,much higher than[(250.8±25.4)U/ml,P<0.05]in the model;the IL-17 and IFN-γmRNA levels in liver tissues in herb-intervened group were(2.1±0.3)and(17.3±3.5),significantly lower than[(3.4±0.5)and(34.5±7.2),P<0.05],whileSOD and FXR mRNA levels were(0.8±0.4)and(2.2±0.5),much higher than[(0.3±0.2)and(1.5±1.1),P<0.05]in the model.Conclusion Yinchen Siling granule could significantly improve liver injuries in rats with cholestatic hepatitis,and the mechanism might be related to its alleviating inflammatory reaction and improve antioxidant capacities by regulating IL-17,IFN-γand other factor expression.