甲状腺穿刺物MiRNAs表达与乳头状甲状腺癌病理特征的关系

The relationship between the 3 types of miRNAs expression in thyroid nodule puncture and the pathology characteristics of papillary thyroid carcinoma

目的研究甲状腺细针穿刺物中miR-221-3p、miR-125b-5p、miR-183-5p的表达及其与乳头状甲状腺癌病理特征的关系.方法2016年12月至2018年10月,青海大学附属医院肿瘤外科二科收住的良性甲状腺结节患者91例,乳头状甲状腺癌34例,术前进行细针穿刺细胞学检查,采用定量PCR对穿刺物进行miR-221-3p、miR-125b-5p、miR-183-5p检测,研究其相对表达量并分析其表达水平与乳头状甲状腺癌病理特征的关系.结果125例甲状腺结节细针穿刺物中miRNA-221-3p、miRNA-125b-5p、miR-183-5p均有表达.miRNA-221-3p表达量良性组1.18±1.09,恶性组4.91±3.64;miRNA-125b-5p表达量良性组1.41±1.35,恶性组2.50±1.28;miR-183-5p表达量良性组1.76±2.79,恶性组2.31±3.01;3种微小核糖核酸表达量恶性组较良性组均显著上调.miRNA-221-3p表达与病灶大小、淋巴结转移及包膜侵犯相关,miRNA-125b-5p与颈部淋巴结转移相关,miR-183-5p与病灶数目和病灶位置相关.结论甲状腺穿刺物中miR-221-3p、miR-125b-5p、miR-183-5p均有表达,miRNA-221-3p、miRNA-125b-5p有助于乳头状甲状腺癌的诊断以及颈部淋巴结转移风险的评估.

Objective Study the relationship between the 3 types of MiRNAs expression in thyroid nodule puncture and the pathology characteristics of papillary thyroid carcinoma.Methods To study the relative expression levels and the relationship between the levels and the pathology characteristics of papillary thyroid carcinoma,91 cases with benign thyroid nodules and 34 cases with papillary thyroid carcinoma from December 2016 to October 2018 are chosen to take thyroid nodule puncture before operation,then test miR-221-3p,miR-125b-5p,miR-183-5p in FNA by using quantitative PCR.Results MiR-221-3p,miR-125b-5p and miR-183-5p are all expressed in the 125 cases.The expression of miR-221-3p is 1.18±1.09 in the benign group and 4.91±3.64 in the malignant group;the expression of miR-125b-5p is 1.41±1.35 in the benign group and 2.50±1.28 in the malignant group;the expression of miR-183-5p is 1.76±2.79 in the benign group and 2.31±3.01 in the malignant group.Compared with the benign group,there is a significant increase in the malignant expression.The expression of miR-221-3p relates to the lesion sizes,lymphatic metastasis and capsular invasion.The expressions of miR-125b-5p relates to cervical lymph node metastasis while the number and location of lesions are related to miR-183-5p.Conclusion MiR-221-3p,miR-125b-5p and miR-183-5p are all expressedin in thyroid nodule puncture.MiR-221-3p and miR-125b-5p is helpful for preoperative diagnosis of papillary thyroid carcinoma and assessment of lymph node metastasis.