福建地区汉族人群中CLDN23基因SNPs多态性与胃癌遗传易感性及预后的相关性

Correlation of CLDN23 SNP Polymorphisms with Hereditary Susceptibility and Prognosis of Gastric Carcinoma in Han Nationality in Fujian

目的探讨福建地区汉族人群中已知的三种claudin-23基因CLDN23单核苷酸多态性(SNPs)与胃癌遗传易感性及预后的相关性。方法应用PCR-LDR法检测CLDN23基因3个SNP位点rs12153、rs1060106 和rs11249884的基因型。结果 CLDN23基因SNP位点rs12153、rs1060106及rs11249884三个位点的基因型及等位基因频率在胃癌病例组和健康者之间差异均无统计学意义(P>0.05);胃癌病例组中三个位点的基因型分布频率与肿瘤分化程度、pT分期显著相关(P<0.05);rs12153、rs1060106的基因型分布频率与患者术后生存时间显著相关(P<0.05);CLDN23基因SNP位点rs12153、rs1060106和rs11249884两两之间未见明显的遗传连锁不平衡性;rs12153、rs1060106和rs11249884可能存在的基因单体型中,C-T-G单体型在胃癌病例组与正常对照组之间有显著差异(P<0.01)。结论福建汉族人群中,CLDN23基因SNP位点rs12153、rs1060106及rs11249884的多态性与胃癌的分化、pT分期具有相关性,可能参与胃癌的进展,rs12153、rs1060106的多态性与胃癌患者的预后有关。

Objective To investigate the correlation of three CLDN23 SNPs with the hereditary susceptibility and prognosis of gastric carcinoma in Han nationality in Fujian.Methods Three SNP loci genotypes(rs12153,rs1060106 and rs11249884)of CLDN23 gene were detected using PCR-LDR method.Results There was no significant difference in genotypes and allele frequencies of CLDN23 SNP rs12153,rs1060106 and rs11249884 between the gastric carcinoma group and the control group(P>0.05).The frequency of genotype distribution in these three loci of the gastric cancer group was significantly correlated with tumor differentiation and pT staging(P<0.05).The genotype distribution frequencies of rs12153 and rs1060106 were significantly correlated with post-operative survival time of the patients(P<0.05).Among rs12153,rs1060106 and rs11249884,linkage disequilibrium of CLDN23 gene was absent.There was significant difference of C-T-G haplotype distribution between the gastric carcinoma group and the control group(P<0.01).Conclusion The polymorphisms of CLDN23 SNP rs12153,rs1060106 and rs11249884 are associated with tumor differentiation and pT staging,suggesting their possible role in the progression of gastric carcinoma in Han nationality in Fujian.The polymorphisms of rs12153 and rs1060106 are correlated with the prognosis of gastric cancer patients.