麦冬药材中6种麦冬皂苷的质量分数测定

Determination of Concentration of 6 Ophiopogons In Ophiopogon Japonicus

建立HPLC法同时测定麦冬药材中penogenin-3-O-α-L-吡喃鼠李糖(1-2)-β-D-葡萄糖苷、去木糖-14-hydro sprengerinin C、麦冬皂苷Ra、penogenin-3-O-α-L-吡喃鼠李糖(1-2)-β-吡喃木糖(1-4)-β-D-葡萄糖苷、14-hydro sprengerinin C和慈溪麦冬皂苷A 6种麦冬皂苷的质量分数。色谱条件为lunna NH2色谱柱(4. 6 mm×150 mm,5μm),流动相乙腈-水(90∶10),流速1. 0m L/min,检测波长208 nm,柱温为室温。该条件下上述6种麦冬皂苷线性范围分别为0. 96~9. 60μg/m L,0. 92~9. 20μg/m L,0. 97~9. 70μg/m L,0. 94~9. 40μg/m L,1. 04~10. 40μg/m L,0. 92~9. 20μg/m L。在相应线性范围内线性相关系数均大于0. 999 1;方法回收率为98. 59%~101. 77%,RSD为1. 15%~1. 98%。所建方法可用于麦冬药材及相关制剂中麦冬皂苷的测定。

A method for determination of concentration of penogenin-3-O-α-L-rhamnopyranosyl(1-2)-β-D-glucopyranoside,dexylopranosyl-14-hydro sprengerininC,ophiopogon Ra,penogenin-3-O-α-Lrhamnopyranosyl(1-2)-β-xylopyranosyl(1-4)-β-D-glucopyranoside,14-hydro sprengerininC,cixiophiopogon A in ophiopogon japonicus was developed by HPLC.The analysis was carried out with lunna NH2 column(4.6 mm×150 mm,5μm),the mobile phase was CH3CN-H2O(90∶10),the flow-rate was 1.0 mL/min.the wave-length was 208 nm and the temperature was room temperature.Under these conditions,the linear range was 0.96~9.60μg/mL,0.92~9.20μg/mL,0.97~9.70μg/mL,0.94~9.40μg/mL,1.04~10.40μg/mL,0.92~9.20μg/mL.The correlation coefficients of 6 ophiopogons were more than 0.9991.The average recoveries of 6 ophiopogons were 98.59%~101.77%with RSD 1.15%~1.98%.The method can be used for determining ophiopogons in Ophiopogon japonicus and related preparations.