2008~2018年捐精志愿者精液细菌培养结果分析

Bacterial culture of donor semen:Analysis of results from 2008 to 2018

目的:分析捐精志愿者精液中病原菌分布情况及细菌感染对精液质量的影响。方法:对2008~2018年4897例捐精志愿者精液样本进行细菌培养,按菌落数不同分为<104 cfu/ml组(n=4229)和≥104 cfu/ml组(n=150),并选择同期未检出细菌的捐精志愿者518例(即菌落数=0 cfu/ml)作为对照组,采用生物梅里埃公司的微量生化反应系统对菌落数≥104 cfu/ml的样本进行菌种鉴定,使用SCA计算机辅助精液分析仪对所有精液样本进行分析,并与对照组的精液质量进行比较。结果:4897例捐精志愿者精液样本细菌培养中,杂菌生长的精液样本6例(0.12%),细菌纯生长的精液样本4379例(89.42%),其中菌落数≥104 cfu/ml的精液样本150例(3.43%)。菌落数≥104 cfu/ml的精液样本中培养出革兰阴性菌(G-菌)104例(69.33%),以大肠埃希菌为主,普通变形杆菌和肠杆菌次之;革兰阳性球菌39例(26.00%),革兰阳性性杆菌4例(2.67%),淋病奈瑟氏菌3例(2.00%)。菌落数≥104 cfu/ml和菌落数<104 cfu/ml的精子总数和前向运动精子百分率明显低于对照组精子总数(P<0.05),精液液化时间、精液pH值、精子总活力和正常形态精子百分率与对照组比较无明显差异(P>0.05)。结论:捐精志愿者精液细菌培养阳性率占89.42%,细菌种类较多,以G-菌为主。同时,精液质量随着菌落数的增多呈降低趋势。

Objective:To investigate the distribution of pathogenic bacteria in donor semen and the effect of bacterial infection on semen quality.Methods:We performed bacterial culture on and counted the bacterial colonies(BC)in the semen samples collected from 4897 sperm donors from 2008 to 2018 and divided them into groups A(BC<104 cfu/ml,n=4229),B(BC≥104 cfu/ml,n=150)and C(BC=0 cfu/ml,n=518).Using the biochemical reaction system of the French Biological Merry Emmanuel Company,we identified the bacterial species in group B,subjected all the semen samples to SCA computer assisted semen analysis,and compared the semen quality among different groups.Results:In the 4897 semen samples,hybrid bacterial contamination was found in 6(0.12%)and non-hybrid bacteria in 4379(89.42%),including 150(3.43%)in group B.In the semen samples with BC≥104 cfu/ml,Gram-negative(G-)bacteria were observed in 104(69.33%),mainly including Escherichia coli,followed by Proteusbacillus vulgaris and Enterobacteria,Gram-positive cocci(G+)in 39(26.00%),G-bacteria in 4(2.67%)and Neisseria gonorrhoeae in 3(2.00%).Compared with group C,groups A and B showed remarkably reduced total sperm count(P<0.05)and percentage of progressively motile sperm(P<0.05)but no statistically significant differences in the semen liquefaction time,semen PH value,total sperm motility or the percentage of morphologically normal sperm(P>0.05).Conclusion:Bacterial culture of donor semen revealed a positive rate of 89.42%and varied the bacterial species,mainly including G-bacteria.And the semen quality decreased with the increase of bacterial colonies.