雄蚕益肾方对LOH大鼠Leydig细胞胆固醇转运蛋白、睾酮合成酶和SF-1表达的影响

Xiongcan Yishen Prescription up-regulates the expressions of cholesterol transport proteins,steroidogenic enzymes and SF-1 in the Leydig cells of rats with late-onset hypogonadism

目的:探讨雄蚕益肾方对LOH大鼠Leydig细胞胆固醇转运蛋白、睾酮合成酶和SF-1表达的影响。方法:25只18月龄雄性SD大鼠随机均分为模型组、丙酸睾酮组、雄蚕益肾方-低、中、高剂量组,2月龄雄性SD大鼠5只设为正常组。雄蚕益肾方-低、中、高剂量组分别给予10.4、20.8、41.6 g/(kg·d)体重剂量的雄蚕益肾方配方颗粒剂灌胃;丙酸睾酮组大鼠肌注5.21 mg/(kg·d).体重剂量的丙酸睾酮,每周3次;正常组和模型组给予相同体积的蒸馏水灌胃,均连续28 d。实验结束后取睾丸组织进行Western印迹检测StAR、TSPO、CYP11A1、HSD3B7、HSD17B4和SF-1蛋白表达。结果:模型组大鼠睾丸组织StAR、TSPO、CYP11A1、HSD3B7、HSD17B4蛋白和SF-1表达均显著低于正常组(P<0.05);与模型组相比,雄蚕益肾方-低、中、高各剂量组大鼠睾丸组织StAR、TSPO、CYP11A1、HSD3B7、HSD17B4蛋白和SF-1显著增高(P<0.05)。结论:雄蚕益肾方能上调LOH大鼠睾丸组织胆固醇转运蛋白(StAR、TSPO)、睾酮合成酶(CYP11A1、HSD3B7、HSD17B4)以及上游转录因子SF-1表达水平,可能是其干预LOH的机制之一。

Objective:To investigate the effects of Xiongcan Yishen Prescription(XYP)on the expressions of cholesterol transport proteins,steroidogenic enzymes and steroidogenic factor-1(SF-1)in the Leydig cells of the rats with late-onset hypogonadism(LOH).Methods:Twenty-five 18-month-old male SD rats were randomly divided into five groups of equal number,LOH model control,testosterone propionate(TP)and low-,medium-and high-dose XYP,and another 5 two-month-old male SD rats included as normal controls.After modeling,the animals in the TP group were treated by intramuscular injection of TP at 5.21 mg/kg qd alt,those in the low-,medium-and high-dose XYP groups intragastrically with XYP at 10.4,20.8 and 41.6 g/kg qd alt respectively,and those in the LOH model and normal control groups with saline,all for 28 successive days.Then,all the rats were sacrificed for determination of the expressions of the cholesterol transport proteins StAR and TSPO,steroidogenic enzymes CYP11A1,HSD3B7 and HSD17B4,and SF-1 in the Leydig cells by Western blot.Results:The expressions of StAR,TSPO,CYP11A1,HSD3B7,HSD17B4 and SF-1 in the Leydig cells were significantly decreased in the LOH model controls compared with those in the normal controls(P<0.05),but remarkably increased in the low-,medium-and high-dose XYP groups in comparison with those in the LOH model control group(P<0.05).Conclusion:Xiongcan Yishen Prescription can up-regulate the expressions of the cholesterol transport proteins StAR and TSPO,steroidogenic enzymes CYP11A1,HSD3B7 and HSD17B4,and SF-1 in the rat Leydig cells,which might be one of the possible mechanisms of the prescription in the treatment of LOH.