微小RNAs对肺癌细胞系侵袭、迁移及凋亡的影响

Effects of microRNAs on invasion,migration and apoptosis of lung cancer cell lines

目的探讨微小RNAs(miRNAs)对肺癌细胞系侵袭、迁移及凋亡的影响。方法通过hsa-mir-933、hsa-mir-4700-3p、hsa-mir-3144-3p、hsa-mir-3972和hsa-mir-548a-5p感染目的细胞,流式细胞术检测5种miRNAs对肺癌细胞系的细胞凋亡的影响;Transwell分析5种miRNAs对肺癌细胞系的细胞侵袭效果;划线分析5种miRNAs对肺癌细胞系的细胞迁移的影响;Real-time PCR检测miRNA表达情况。结果与感染空载体组和无感染的对照组相比,感染了hsa-mir-933、hsa-mir-4700-3p、hsa-mir-3144-3p、hsa-mir-3972和hsa-mir-548a-5p的细胞凋亡明显增加,其中NCI-H460和A549均以转染hsa-mir-4700-3p凋亡率最高;5种miRNAs对肺癌细胞的侵袭数目明显减小;5种miRNAs对肺癌细胞的细胞的迁移效果减弱;Real-time PCR检测hsa-mir-933、hsa-mir-4700-3p、hsa-mir-3144-3p、hsamir-3972和hsa-mir-548a-5p处理后相应miRNA的水平表达均相应增加。结论5种miRNAs能够促进肺癌细胞系的凋亡,降低肺癌细胞系的高侵袭性,抑制肺癌细胞系的迁移,增加对应miRNA的表达。

Objective To investigate the effects of microRNAs(miRNAs)on invasion,migration and apoptosis of lung cancer cell lines.Methods Target cells were infected with hsa-mir-933,hsa-mir-4700-3p,hsa-mir-3144-3p,hsamir-3972,and hsa-mir-548a-5p.Cell apoptosis was measured using flow cytomertry,Transwell invasion assay,cell migration were analyzed by lineation,miRNA was quantified using Real-time PCR.Results Compared with the vector and control,in hsa-mir-933,hsa-mir-4700-3p,hsa-mir-3144-3p,hsa-mir-397 and hsa-mir-548a-5p group the apoptosis significantly increased,cell invasion ability significantly decreased,cell migration ability significantly decreased,miRNAs expression significantly increased.Conclusion Five microRNAs can promote the apoptosis of lung cancer cell lines,change the high invasiveness of lung cancer cell lines,inhibit the migration of lung cancer cell lines,and increase the expression of corresponding miRNAs.