人包皮组织脱细胞基质的制备与生物相容性

Preparation and biocompatibility of acellular matrix grafts of human foreskin

目的制备人包皮组织脱细胞基质移植物(AMGs),探讨其浸提液对兔尿道黏膜细胞毒性。方法制备人包皮组织脱细胞基质后,通过Masson染色观察脱细胞后基质的表征,扫描电子显微镜观察其孔径大小。体外培养尿道黏膜细胞,采用免疫荧光鉴定其AE3表达。尿道黏膜细胞与AMGs基质浸提液体外共培养,MTT法检测AMGs浸提液对尿道黏膜细胞毒性。结果Masson染色显示细胞去除干净,只有胶原蛋白构成的支架,扫描电镜显示支架的孔径为20~50μm,分布均匀,形态良好。适当浓度的AMGs基质浸提液对尿道黏膜细胞无明显毒性。结论人包皮组织脱细胞基质为理想的AMGs支架材料,制备的AMGs材料细胞毒性小,有一定的实用价值。

Objective To prepare human foreskin tissue acellular matrix graft(AMGs),and explore the toxicity of the extracts on rabbit urethral mucosal cells.Methods After preparing the decellularized matrix of human foreskin tissue,the characteristics of decellularized matrix were observed by Masson staining,and the pore size was observed by scanning electron microscope.Results Masson staining showed that the cells were removed,and the scaffold was composed of collagen.Scanning electron microscopy showed that the pore size of the scaffold was about 20-50μm,which was uniformly distributed.The appropriate concentration of AMGs matrix extract had no obvious toxicity to urethral mucosal cells.Conclusion The acellular matrix of human foreskin tissue is an ideal AMGs scaffold material.The prepared AMGs material has low cytotoxicity and has practical value.