油墨直写打印纳米羟基磷灰石/聚乳酸复合材料:制备及性能研究

Preparation and Properties of Ink Direct Writing Nano-hydroxyapatite/Polylactic Acid Composite Materials

油墨直写打印(Direct ink writing,DIW)是一种基于浆料挤出的增材制造技术,其操作简单,成本低,可用于生物支架的制备和成形。本试验通过在纳米羟基磷灰石/聚乳酸(HA/PLA)的复合材料中添加10%海藻酸钠(Alg)作交联剂以支撑成形件,并对复合材料及DIW成形件的物理、化学、生物学性能进行了评价。结果表明:DIW成形件的最佳后处理温度80℃,拉伸试样的伸长率为0.06%,拉伸强度为0.5MPa。复合材料在DIW打印成形和后处理过程后仍然具有PLA和HA材料的良好生物相容性和骨诱导性。在细胞毒性试验中,DIW成形件和压片成形件的细胞数量均呈现显著增长趋势,表现为无明显毒性。由此证明油墨直写打印成形此生物可降解材料是可行的,后续可进行优化提高DIW成形件的机械性能。

Direct ink writing is an additive manufacturing technology based on slurry extrusion.It is easy to operate and low-cost,and can be used in the preparation and forming of biological scaffolds.10%Alg is added to HA/PLA composites as a crosslinking agent to support the DIW parts,and then the physical,chemical and biological properties of the composites and the DIW parts are evaluated.The results show that the optimal post-treatment temperature of the DIW part is 80℃,the elongation of the tensile specimen is 0.06%,and the tensile strength is 0.5 MPa.The composite material still had good biocompatibility and osteoinductivity of PLA and HA after the DIW process and post-treatment processes.In the cytotoxicity experiment,the number of cells in the DIW parts and tablet-formed parts exhibited a significant growth trend which indicated no obvious toxicity.It is demonstrated that it is feasible to form this biodegradable material by direct ink writing.And the mechanical properties of the DIW parts can be improved through some optimization at a later stage.