Cu(Ⅱ)对一株镰刀菌形态发育及木质素酶系活性的影响

Induction of Cu(Ⅱ)on morphological development and ligninase activities of Fusarium sp.ZH-H2

本试验采用室内摇瓶培养,通过添加不同浓度的Cu(Ⅱ),研究了Cu(Ⅱ)对降解菌Fusarium sp.ZH-H2形态发育及木质素酶系活性的影响,以期诱导提高木质素酶系的活性。结果表明,当Cu(Ⅱ)浓度为0.50mmol/L时,菌丝生物量最高达0.38g,菌丝团呈洁白平滑球状且数量最多,此浓度下木质素过氧化物酶(lignin peroxidase,LiP)活性峰值也达到最高,为28315.41U/L,当Cu(Ⅱ)浓度为2.00mmol/L时,有利于诱导锰过氧化物酶(manganese peroxidase,MnP)和漆酶(laccase,Lac)的代谢,最高峰值分别为1612.90U/L、7829.12U/L,并且明显缩短了峰值的形成时间,浓度过高或过低则对3种木质素酶系活性产生抑制效应,为下一步分子生物学研究奠定了基础。

Shake flask culture was used to study the effects of different concentration of Cu(Ⅱ)on the morphological development and ligninase activity of Fusarium sp.ZH-H2.The results showed that when Cu(Ⅱ)concentration was 0.50mmol/L,the highest mycelial biomass was up to 0.38g,and the mycelia were white,smooth and spherical and reached the maximum in number.At this concentration,the peak value of lignin peroxidase(LiP)activity reached 28351.41U/L.Cu(Ⅱ)concentration of 2.00mmol/L was beneficial to induce the metabolism of manganese peroxidase(MnP)and laccase(Lac).The highest peak value of MnP and Lac were 1612.90U/L and 7829.12U/L,respectively,and the peak formation time was significantly shortened.Excessively high or low concentration brought about inhibitory effect on the activity of the three lignin enzymes.