响应面法优化蛹虫草与厚朴双向液体发酵工艺

Response surface methodology optimizing liquid fermentation process of Cordyceps militaris by use of magnoliae officinalis cortex as additional substrate

为提高蛹虫草液体发酵胞外多糖含量和菌丝体生物量,以厚朴为药性基质,对蛹虫草进行双向液体发酵。在单因素实验的基础上,应用Box-Behnken实验设计,对发酵过程关键影响因素进行优化。结果表明,在厚朴添加量5g、接种量15.5mL、发酵温度25℃的条件下发酵9d,蛹虫草双向液体发酵产物中胞外多糖含量为3.11mg/mL,菌丝体生物量为18.81mg/mL。各发酵因素中,发酵液胞外多糖含量受接种量影响最大,菌丝体生物量则主要受发酵温度影响。优化所得发酵工艺可行性高、周期短、生产过程可控,为进一步提高人工培育蛹虫草质量、增加其关键活性产物的产量提供了参考。

Liquid fermentation of Cordyceps militaris was carried out using chinese medicine magnoliae officinalis cortex as additional substrate.Based on single factor experiment,the key influencing factors of the fermentation process were optimized by Box-Behnken experimental design.The results showed that the extracellular polysaccharide content and mycelial biomass of C.militaris liquid fermentation products were 3.11mg/mL and 18.81mg/mL,respectively,under the addition of magnoliae officinalis cortex of 5g and optimized fermentative condition including inoculum volume of 15.5mL,fermentation temperature of 25°C and fermentative duration of 9d.It was indicated that the content of extracellular polysaccharide in fermentation broth was obviously affected by inoculum quantity,and the biomass of mycelia was mainly affected by fermentation temperature.The optimized process is feasible,short-cyclic and controllable.