非特异性酯酶及酸性和碱性磷酸酶在奥尼罗非鱼消化道的分布

Distribution of Nonspecific Esterase,Acid Phosphatase and Alkaline Phosphatase in Digestive Tract of Tilapia Galilaea

本研究利用石蜡切片结合组织化学方法研究奥尼罗非鱼(Oreochromis niloticus♀×O.aureus♂)消化道内非特异性酯酶、酸性磷酸酶和碱性磷酸酶的分布特点。结果发现,非特异性酯酶大量密集分布在前肠,其次为胃、食管和中肠,极少数零散分布在后肠。酸性磷酸酶主要分布于奥尼罗非鱼前肠,其次为中肠和胃,极少数分布在食管和后肠。碱性磷酸酶大量密集分布在奥尼罗非鱼前肠和中肠,其次为胃、食管和后肠。结果表明,奥尼罗非鱼前肠、中肠、食管和胃有较强的吸收和消化脂质功能,前肠、中肠和胃是蛋白质吸收和消化的主要部位,前肠和中肠是营养物质吸收的主要部位。

The distributions of nonspecific esterase,acid phosphatase and alkaline phosphatase in digestive tract of Tilapia Galilaea(Oreochromis niloticus♀×O.aureus♂)were studied by paraffin section combined with histochemical methods including Gomori’s staining and acid-α-naphthyl-acetate esterase staining.It was found that nonspecific esterase densely distributed in the foregut,massively distributed in the stomach,esophagus and midgut,and slightly distributed in the hindgut(Fig.1).Acid phosphatase mainly distributed in the foregut,followed by the midgut and stomach,and few in the esophagus and hindgut(Fig.2).Alkaline phosphatase was densely observed in the foregut and midgut,and a larger amount was also observed in the stomach,esophagus and hindgut(Fig.3).The results showed that the foregut,midgut,esophagus and stomach of Tilapia Galilaea were the main sites of lipid absorption and digestion.The foregut,midgut and stomach were the main sites of protein absorption and digestion,and the foregut and midgut were the main sites of nutrient absorption.