等温扩增技术的原理及应用

Principle and application of isothermal amplification technology

等温扩增技术是在恒温条件下进行扩增的一类新型核酸扩增技术。基于该技术建立的方法操作简便,且具有较高的灵敏度与特异性,在临床与科研等方面展现了较好的应用前景。本文就环介导等温扩增、交叉引物扩增、链替代扩增、依赖核酸序列的等温扩增和滚环扩增等技术的原理、特点及应用进行了综述。

Isothermal amplification technology is a new class of nucleic acid amplification technology that performs amplification under constant temperature conditions.With the advantages of simple operation,high sensitivity and specificity,this kind of technology shows a good application prospect in clinical detection and scientific research.The principle,characteristics and applications of loop-mediated isothermal amplification(LAMP),crossing priming amplification(CPA),strand displacement amplification(SDA),recombinase polymerase amplification(RPA),nucleic acid sequence-based amplification(NASBA),rolling circle amplification(RCA)and helicase-dependent amplification(HDA)are reviewed in this paper.