黄草乌内生真菌接骨木镰孢菌B10.2次生代谢产物研究

Secondary metabolites of Fusarium sambucinum B10.2 from Aconitum vilmorinianum Kom.

本文对分离自黄草乌(Aconitum vilmorinianum Kom.)的一株接骨木镰孢菌(Fusarium sambucinum B10.2)的次生代谢产物进行了研究,从中分离鉴定了10个化合物:cyclonerodiol(1)、trichoderiol A(2)、T-2 tetraol(3)、sambacide(4)、12β-acetoxy-4,4-dimethyl-24-methylene-5α-cholesta-8,14-diene-3β,11α-diol(5)、bisphenol A(6)、β-谷甾醇(7)、木兰脂素(8)、吲唑(9)、尿嘧啶(10),1~3和5~10均是首次从接骨木镰孢菌中分离得到,其中6首次从天然产物中发现。活性研究表明6和9对金黄色葡萄球菌具有较强的抑制活性,其最小抑菌浓度(MIC)与阳性对照相同,为64μg/mL;4对大肠杆菌的生长具有一定的抑制作用,最小抑菌浓度(MIC)为32μg/mL;2、3和4具有中等强度的乙酰胆碱酯酶抑制活性。

Ten compounds were obtained from Fusarium sambucinum B10.2,which was isolated from Aconitum vilmorinianum Kom.Their structures were identified as:cyclonerodiol(1),trichoderiol A(2),T-2 tetraol(3),sambacide(4),12β-acetoxy-4,4-dimethyl-24-methylene-5α-cholesta-8,14-diene-3β,11α-diol(5),bisphenol A(6),β-sitosterol(7),magnolin(8),indazole(9),uracil(10).Compounds 1-3,5-10 were isolated from this fungus for the first time,and 6 was first obtained from nature.The results of bioactivity assays showed that compound 4 could inhibit the growth of Escherichia coli with the minimum inhibitory concentration(MIC)of 32μg/mL.Compounds 6 and 9 showed strong inhibitory activity against Staphylococcus aureus,and the MIC was 64μg/mL,which was at the same grade as positive control.Compounds 2,3 and 4 exhibited moderate acetylcholinesterase(AChE)inhibitory effects.