摘要
建立UPLC-ESI-IT-TOF-MS法测定血府逐瘀汤中苦杏仁苷、羟基红花黄色素A、甘草苷、芦丁、芸香柚皮苷、柚皮苷、新橙皮苷和柴胡皂苷A 8个成分含量的方法。采用Shim-Pack XR-ODS C18色谱柱(2.0 mm×75 mm,1.6μm),以水/甲酸(0.1%)-乙腈为流动相,梯度洗脱;采用ESI离子源,正负离子模式同时采集,IT-TOF-MS检测器定性定量测定。结果表明,8个成分的线性关系、日内和日间精密度、稳定性均良好,加样回收率在89.8%~108.1%范围内。本实验方法简单、灵敏,充分发挥了IT-TOF-MS检测器稳定性好、质量数精确、分辨率高、准分子离子外标法定量准确的优势,为血府逐瘀汤的质量控制提供科学依据。
To establish a UPLC-ESI-IT-TOF-MS method for simultaneous determination of amygdalin,hydroxysafflor yellow A,liquiritin,rutin,narirutin,niringin,neohesperidin and saikosaponin A in Xuefu Zhuyu Decoction.The chromatographic separation was on Shim-Pack XR-ODS C 18 column(2.0 mm×75 mm,1.6μm)with a mobile phase consisting of water/formic acid(0.1%)-acetonitrile and gradient elution.ESI ionization source,combined with positive and negative scanning switch simultaneously,and IT-TOF-MS was used for qualitative and quantitative analysis.The results indicated that the linear relationship,intra-day and inter-day precision,and stability of the eight components were all good,and the average recoveries of the eight components were in the range of 89.8%-108.1%.This experimental method is simple and sensitive.which gives full play to the advantages of the IT-TOF-MS detector with good stability,accurate mass,high resolution,and accurate quantification by the external standard method of the excimer ion,and can be expected to provide a scientific basis for quality control of Xuefu Zhuyu Decoction.
作者
傅春燕
王惠余
刘永辉
王杨
FU Chun-yan;WANG Hui-yu;LIU Yong-hui;WANG Yang(College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China;College of Pharmacy and College of Food and Chemical Engineering,Shaoyang University;The Second Affiliated Hospital,Shaoyang University,Shaoyang 422000,China;Institute of Integrated Traditional Chinese and Western Medicine,Xiangya Hospital,Central South University,Changsha 410008,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第6期1006-1013,共8页
Natural Product Research and Development
基金
国家自然科学基金(21675174,21873116,81673719)
2018年度湖南省技术创新引导计划临床医疗技术创新引导项目(2018SK51902)。
