甘肃各主产区当归UPLC多成分含量测定及评析

Determination and evaluation of multicomponent content of Angelica sinensis in the main production areas of Gansu by UPLC

本研究建立了UPLC同时测定甘肃省各产区当归中8个指标成分含量的方法,采用Waters ACQUITY UPLC^■BEH C18色谱柱(2.1 mm×50 mm,1.7μm),流动相甲醇-1%乙酸水,梯度洗脱,体积流量0.3 mL/min,柱温30℃,检测波长270 nm。经方法学验证,重复性、稳定性、精密度、加样回收率RSD均小于3%,8个指标成分在各自范围内线性关系良好,相关系数(R^2)大于0.999。结合主成分分析对不同产区当归综合品质进行研究,主成分分析结果显示定西市各产区当归总体质量优于其他各市,另外绿原酸、洋川芎内酯A、藁本内酯含量在不同产地样品中存在较大波动,对当归的质量有较大影响,以上研究结果为甘肃省当归的质量评价提供了理论基础。

In this work,an UPLC method was developed for the simultaneous determination of 8 components in Angelica sinensis in Gansu Province.Chromatographic separation was performed on a Waters ACQUITY UPLC^■ BEH C 18 column(2.1 mm×50 mm,1.7μm)through a gradient delivery of a mixture of methanol and 1%acetic acid at a flow rate of 0.3 mL/min.The column temperature was maintained at 30℃,and the detection wavelength was set at 270 nm.The results of method validation showed that the RSDs of reproducibility,stability,precision and recovery were all less than 3%,and all of the analytes exhibited good linearity(R^ 2>0.999)in a relatively wide concentration range.Principal component analysis was further applied to study the comprehensive quality of Angelica sinensis in different producing areas and the results suggested that the quality of Angelica sinensis in Dingxi City was better than that in other cities.In addition,the contents of chlorogenic acid,senkyunolide A and ligustilide fluctuated greatly for different areas,which had great influence on the quality of Angelica sinensis.The obtained findings provided a theoretical basis for the quality evaluation of Angelica sinensis in Gansu Province.