湖南烟草病毒病种类检测与系统进化分析

Detection and Phylogenetic Analysis of Tobacco Viruses in Hunan Province

为明确湖南烟草病毒病的种类及分布状况,利用小干扰RNA(si RNA)测序和反转录PCR(RT-PCR)对湖南烟区样品进行检测,分析主要病毒侵染类型,最大似然法构建系统进化树确定分类地位。结果表明,湖南烟草受到烟草普通花叶病毒(Tobacco mosaic virus,TMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、马铃薯Y病毒(Potato virus Y,PVY)、番茄斑驳花叶病毒(Tomato mottle mosaic virus,To MMV)、地黄花叶病毒(Rehmannia mosaic virus,Re MV)和辣椒轻斑驳病毒(Pepper mild mottle virus,PMMV)等6种病毒的侵染,其中To MMV、Re MV、PMMV首次被检测到,TMV、CMV和PVY是主要病毒,TMV为优势病毒,复合侵染是主要侵染类型,复合侵染率为71.29%;湖南烟草上的TMV分离物形成3个独立组系,CMV分离物属群组I的亚组IB,PVY分离物属PVYNTN-a、PVYE和PVYSYR-I株系。本研究摸清了湖南烟草病毒种类、分布及侵染情况,明确了TMV、CMV、PVY的分类地位,为病毒病的防治提供了理论依据。

Small interfering RNA(siRNA)and RT-PCR were used to detect the samples for determine the species and distribution of tobacco virus diseases in Hunan province,and the main virus infection types and the taxonomic status were clarified.The results showed that there were Tobacco mosaic virus(TMV),Cucumber mosaic virus(CMV),Potato virus Y(PVY),Tomato mottle mosaic virus(TMV),Rehmannia mosaic virus(ReMV)and Pepper mild mottle virus(PMMV).Among them,ToMMV,ReMV and PMMV were tested in the first time in Hunan tobacco.TMV,CMV and PVY were the main viruses,and TMV was the dominant virus species,the mix-infection rate was 71.29%.TMV isolates in Hunan tobacco area formed three relatively independent populations,CMV isolates belonged to subgroup IB of group I,and PVY isolates belong to PVYNTN-a,PVYE and PVYSYR-I.In this study,the species,distribution and infection type of tobacco virus in Hunan tobacco area were found out,and the taxonomic status of TMV,CMV and PVY were clarified,which provided a theoretical basis for the prevention and control of tobacco virus diseases.