生物酶电化学传感器在有机磷农药检测的研究进展

Research Progress in Electrochemical Biosensors Based on Bio-enzyme for Detection of Organophosphorus Pesticides

有机磷农药在农业和生活中应用非常广泛,因而持续存在于水源、水果、蔬菜和加工食品中,对人类健康和环境造成极大的危害.因此,建立快速便捷、高灵敏度和高选择性的有机磷农药检测方法是非常有必要的.生物传感检测技术能够满足有机磷农药分析的需要,得到了广泛的应用.本文综述了多种电化学生物传感器检测有机磷农药的原理和方法,通常包括抑制酶电极和催化反应酶电极,抑制酶电极所采用的生物酶包括乙酰胆碱酯酶(AChE)、丁酰胆碱酯酶、酪氨酸酶和碱性磷酸酶等,反应酶电极主要采用有机磷酸水解酶.通过对比表明,基于AChE抑制酶电极的电流生物传感器具有最高的灵敏度,其线性范围在1.0×10-11~1.0×10-2μmol·L-1,检测限能够达到1×10-11μmol·L-1.

Organophosphorus(OP)pesticides have been used widely as agricultural and household pest control agents and persist in our water resources,fruits,vegetables and processed food as health and environmental hazardous compounds.Thus,detection of these harmful OP pesticides at an ease with high sensitivity and selectivity is necessary.Bio-sensing technology meets these requirements and has been employed at a large scale for detection.The review describes the principle and strategy of various electrochemical OP biosensors for detection of OP pesticides in detail.The electrochemical biosensors are generally,based on inhibition of enzyme,acetyl cholinesterase(AChE),butyryl cholinesterase(BChE),tyrosinase and alkaline phosphatase or enzyme(organophosphorus hydrolase,OPH)catalyzed reaction.The detection limits and linearity range of various OP biosensors have also been compared.AChE inhibition based amperometric OP biosensorsexhibited the lowest detection limit of 1×10-11μmol·L-1 with a linearity range of 1.0×10-11~1.0×10-2μmol·L-1.