白灵菇根部抗癌活性多糖的分离纯化和结构分析

Isolation,Purification and Structure Analysis of Anticancer Active Polysaccharides from Pleurotus nebrodensis Roots

采用水提醇沉、DEAE-纤维素-52和Sepharose 2B柱层析等方法提取、纯化白灵菇根部抗癌活性多糖,得到抗癌活性组分PN75-2;采用MTT法研究PN75-2多糖组分对HepG-2细胞增殖的抑制作用;采用高效液相色谱(HPLC)、傅里叶变化红外光谱(FTIR)、气相色谱(GC)、高效凝胶渗透色谱(HPGPC)以及核磁共振(NMR)等技术对多糖组分PN75-2进行结构分析。结果表明:PN75-2多糖相对分子质量为290 kDa,其单糖组成主要为甘露糖、葡萄糖和半乳糖,并含有微量木糖及痕量鼠李糖和阿拉伯糖;PN75-2对HepG-2细胞增殖有较强的抑制作用,在浓度为200μg/mL时,抑制率为38.3%。研究结果可为白灵菇根部多糖在功能性食品或药品开发中的应用提供参考。

Anticancer active polysaccharides were isolated from root of Pleurotus nebrodensis by water extraction and alcohol precipitation and further fractionated on DEAE-cellulose-52 and Sepharose 2B chromatography columns to afford active polysaccharide fraction PN75-2.The inhibitory effect of the polysaccharide fraction PN75-2 on HepG-2 cells proliferation was studied by MTT method and structure of PN75-2 was analyzed by high performance liquid chromatography(HPLC),fourier transform infrared spectroscopy(FTIR),gas phase chromatography(GC),high performance gel permeation chromatography(HPGPC)and nuclear magnetic resonance(NMR).The results showed that,the relative molecular weight of PN75-2 was 290 kDa.The monosaccharide consisted mainly of mannose,glucose and galactose,and micro amounts of xylose,trace amounts of rhamnose and arabinose.PN75-2 showed a strong inhibitory effect on HepG-2 cell proliferation,with an inhibition rate of 38.3%at a concentration of 200μg/m L.The results could provide a reference for the application of Pleurotus nebrodensis polysaccharides in the development of functional foods or pharmaceuticals.